Further cholinergic aspects of carotid body chemotransduction of hypoxia in cats.
Further cholinergic aspects of carotid body chemotransduction of hypoxia in cats.
复制标题
猫缺氧颈动脉体化学转导的进一步胆碱能方面。
DOI：
10.1152/jappl.1997.82.3.819
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Ide,T
中科院分区：
文献类型：
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作者：
Fitzgerald,RS;Shirahata,M;Ide,T
罗伯特·S·菲茨杰拉德，白畑真子，井透鲁。猫颈动脉缺氧体化学转导的胆碱能方面研究。达成。生理学报，32(3):819-827,1997。从20世纪30年代到70年代，乙酰胆碱（ACh）在颈动脉体缺氧化学转导中的作用一直存在争议。自20世纪70年代末以来，这个问题只是断断续续地或根本不被关注。本研究的目的是用新的制备方法再次验证乙酰胆碱在猫颈动脉体缺氧的化学转导中是一种兴奋性神经递质的假设。我们测试了特定的烟碱阻滞剂甲美胺和所有五种毒蕈碱受体的毒蕈碱阻滞剂阿托品的效果。我们进一步测试了m1和m2毒蕈碱受体阻滞剂的作用。颈动脉体区选择性灌注无阻滞剂或含有不同剂量阻滞剂的低氧克雷布斯-林格碳酸氢盐（KRB）溶液。甲美胺和阿托品均以剂量相关的方式降低了对缺氧KRB的反应。m2毒蕈碱受体阻滞剂胆碱胺和AFDX 116增加了对缺氧KRB的反应，而m1毒蕈碱受体阻滞剂吡仑西平降低了对缺氧KRB的反应。这些数据与乙酰胆碱在猫颈动脉缺氧体化学转导中的兴奋作用一致。
Fitzgerald, Robert S., Machiko Shirahata, and Tohru Ide.Further cholinergic aspects of carotid body chemotransduction of hypoxia in cats.J. Appl. Physiol.82(3): 819–827, 1997.—From the 1930s into the 1970s, the role of acetylcholine (ACh) in the carotid body’s chemotransduction of hypoxia was debated. Since the late 1970s, the issue has been pursued only intermittently or not at all. The purpose of this study was to test again with a new preparation the hypothesis that ACh is an excitatory neurotransmitter in the cat carotid body’s chemotransduction of hypoxia. We tested the effect of the specific nicotinic blocker mecamylamine and the muscarinic blocker of all five muscarinic receptors, atropine. We further tested the effects of M1and M2muscarinic-receptor blockers. The carotid body region was selectively perfused with hypoxic Krebs-Ringer bicarbonate (KRB) solutions that were blocker free or contained varying doses of the blockers. Both mecamylamine and atropine reduced the response to hypoxic KRB in a dose-related manner. The M2muscarinic-receptor blockers gallamine and AFDX 116 increased the response to hypoxic KRB, whereas the M1muscarinic-receptor blocker pirenzepine reduced the response to hypoxic KRB. These data are consistent with an excitatory role for ACh in the carotid body chemotransduction of hypoxia in the cat.