Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice.
Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice.
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DOI：
10.1111/jipb.12336
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发表时间：
2015-09
影响因子：
11.4
通讯作者：
Dekai Wang;Heqin Liu;Sujuan Li;Guowei Zhai;Jianfeng Shao;Y. Tao
中科院分区：
文献类型：
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作者：
Dekai Wang;Heqin Liu;Sujuan Li;Guowei Zhai;Jianfeng Shao;Y. Tao
丝氨酸羟甲基转移酶（Serine hydroxymethyltransferase，SHMT）是高等植物一碳代谢和光呼吸的重要酶，参与植物的生长发育和抗生物及非生物胁迫。利用图位克隆技术克隆了水稻丝氨酸羟甲基转移酶基因OsSHM1，OsSHM1是拟南芥SHM1的直系同源基因。osshm1突变体有褪绿病变和一个相当小的，致命的表型在自然环境中的CO2浓度，但可以恢复到野生型与正常增长下升高的CO2水平（0.5%CO2），表现出典型的光呼吸表型。抗氧化酶活性测定结果表明osshm1受到了明显的氧化应激。此外，OsSHM1在所有测试的器官（根，秆，叶和幼穗）中表达，但主要在叶中。OsSHM1蛋白定位于线粒体。我们的研究表明OsSHM1基因的分子功能在水稻和拟南芥中是保守的。
Serine hydroxymethyltransferase (SHMT) is important for one carbon metabolism and photorespiration in higher plants for its participation in plant growth and development, and resistance to biotic and abiotic stresses. A rice serine hydroxymethyltransferase gene, OsSHM1, an ortholog of Arabidopsis SHM1, was isolated using map-based cloning. The osshm1 mutant had chlorotic lesions and a considerably smaller, lethal phenotype under natural ambient CO2 concentrations, but could be restored to wild type with normal growth under elevated CO2 levels (0.5% CO2 ), showing a typical photorespiratory phenotype. The data from antioxidant enzymes activity measurement suggested that osshm1 was subjected to significant oxidative stress. Also, OsSHM1 was expressed in all organs tested (root, culm, leaf, and young panicle) but predominantly in leaves. OsSHM1 protein is localized to the mitochondria. Our study suggested that molecular function of the OsSHM1 gene is conserved in rice and Arabidopsis.