Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus
Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus
复制标题
生成 MERTK 基因座的纯合 CRISPR/Cas9 介导的敲除 H9 hESC 亚系
DOI：
10.1016/j.scr.2021.102310
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发表时间：
2021
影响因子：
1.2
通讯作者：
Yizhi Liu
中科院分区：
文献类型：
--
作者：
Xiangcheng Tang;Jia-ling Fu;Zhigang Chen;Lixia Luo;David Wan-Cheng Li;Yizhi Liu
MERTK突变与视杆-视锥营养不良相关。为了能够研究这种疾病的机制，我们产生了H9人胚胎干细胞的细胞系资源，其在MERTK基因的外显子19中具有纯合状态的大片段缺失突变。该亚系表达多能干细胞标志物，呈现正常核型，并保留分化成内胚层、中胚层和外胚层谱系的能力。
MERTK mutations are associate with rod-cone dystrophies. To enable investigations into the mechanism of this disease, we generated a cell line resource of H9 human embryonic stem cells harboring large fragment deletion mutation in a homozygous state in exon 19 of the MERTK gene. This subline expressed pluripotent stem cell markers, presented a normal karyotype, and preserved the ability to differentiate into endodermal, mesodermal, and ectodermal lineages.