Screening of Diatom Strains and Characterization of Cyclotella cryptica as A Potential Fucoxanthin Producer.
Screening of Diatom Strains and Characterization of Cyclotella cryptica as A Potential Fucoxanthin Producer.
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DOI：
10.3390/md14070125
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发表时间：
2016-07-08
期刊：
影响因子：
5.4
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Guo B;Liu B;Yang B;Sun P;Lu X;Liu J;Chen F
岩藻黄素由于其广泛的健康有益作用而受到越来越多的关注。目前，海藻是天然岩藻黄质的主要来源。然而，岩藻黄质含量低得令人沮丧，阻碍了它们的使用，推动了对替代岩藻黄质生产商的探索。在本研究中，13硅藻菌株进行了评价方面的增长和岩藻黄质的生产潜力。隐小环藻（CCMP 333），生长良好的岩藻黄质的生产在光合自养和异养生长条件下，被选为进一步研究。硝酸盐和光照单独或组合供应都被发现促进生长和岩藻黄素积累。当将异养培养物转移到光时，岩藻黄质对光强度的反应不同，并且受到较高光强度的损害，伴随着硅甲藻黄质和硅藻黄质的增加，指示硅甲藻黄质循环的调制以应对光胁迫。本研究首次对具有产岩藻黄质潜力的硅藻菌株进行了筛选，并详细研究了营养和环境因素对岩藻黄质产量的影响。隐藻生长和岩藻黄素积累。这些结果为C. cryptica培养参数用于提高岩藻黄质产量和C.隐藻可能成为岩藻黄质的一种有前途的微藻来源。
Fucoxanthin has been receiving ever-increasing interest due to its broad health beneficial effects. Currently, seaweeds are the predominant source of natural fucoxanthin. However, the disappointingly low fucoxanthin content has impeded their use, driving the exploration of alternative fucoxanthin producers. In the present study, thirteen diatom strains were evaluated with respect to growth and fucoxanthin production potential. Cyclotella cryptica (CCMP 333), which grew well for fucoxanthin production under both photoautotrophic and heterotrophic growth conditions, was selected for further investigation. The supply of nitrate and light individually or in combination were all found to promote growth and fucoxanthin accumulation. When transferring heterotrophic cultures to light, fucoxanthin responded differentially to light intensities and was impaired by higher light intensity with a concomitant increase in diadinoxanthin and diatoxanthin, indicative of the modulation of Diadinoxanthin Cycle to cope with the light stress. Taken together, we, for the first time, performed the screening of diatom strains for fucoxanthin production potential and investigated in detail the effect of nutritional and environmental factors on C. cryptica growth and fucoxanthin accumulation. These results provide valuable implications into future engineering of C. cryptica culture parameters for improved fucoxanthin production and C. cryptica may emerge as a promising microalgal source of fucoxanthin.