The major glycerophospholipids of the predatory and parasitic bacterium Bdellovibrio bacteriovorus HID5.
The major glycerophospholipids of the predatory and parasitic bacterium Bdellovibrio bacteriovorus HID5.
复制标题
捕食性和寄生性细菌噬菌弧菌 HID5 的主要甘油磷脂。
DOI：
10.1007/s11745-008-3235-9
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发表时间：
2008
期刊：
影响因子：
1.9
通讯作者：
Kaneshiro,EdnaS
中科院分区：
文献类型：
--
作者：
Nguyen,Nhu-AnT;Sallans,Larry;Kaneshiro,EdnaS
蜈蚣弧菌是攻击和寄生较大革兰氏阴性细菌的小型可移动细菌，由于它们可能具有实际应用，这些微生物正引起研究人员的注意，如theb的测序所表明的那样。细菌基因组。有一份早些时候的报告显示。细菌在寄生阶段从宿主细胞中清除脂肪酸，否则其脂类的生化性质，特别是其复杂的脂类，仍然未知。我们在这里报告了一株轴向培养的非寄主非依赖性菌株(HID5)的磷脂类别。磷脂和脂肪酸通过各种色谱程序和高分辨率质谱学技术进行鉴定。磷脂酰乙醇胺是主要的磷脂，还鉴定了磷脂酰丝氨酸、心磷脂、磷脂酰甘油和N-酰基磷脂酰乙醇胺。主要脂肪酸为16：0、16：1、18：1和9，10-Mt C16：0(cyC17：0)。与另一种捕食性细菌UKI2不同的是，在INB中没有检测到鞘磷脂。通过本研究中使用的程序进行细菌培养。这与b中明显缺乏编码鞘脂生物合成酶的基因是一致的。细菌基因组数据库。这一结果与蛭弧菌和杆菌属的分离结果一致。
Bdellovibrioare small motile bacteria that attack and parasitize larger Gram-negative bacteria and since they might have practical applications, these organisms are attracting the attention of researchers as indicated by the sequencing of theB. bacteriovorusgenome. There is an earlier report showing thatB. bacteriovorusscavenges fatty acids from the host cell during its parasitic phase otherwise the biochemical nature of its lipids, particularly its complex lipids, remains unknown. We here report on the phospholipid classes of an axenically cultured host-independent strain (HID5). Phospholipids and fatty acids were identified by a variety of chromatographic procedures and high-resolution mass spectrometric techniques. Phosphatidylethanolamine was the major phospholipid and phosphatidylserine, cardiolipin, phosphatidylglycerol, andN-acylphosphatidylethanolamine were also identified. The major fatty acids were 16:0, 16:1, 18:1, and 9,10-Mt C16:0 (cyC17:0). Unlike another predatory bacterium,Bacteriovorus stolpiistrain UKi2, sphingolipids were not detected inB. bacteriovorusby the procedures used in this study. This is consistent with the apparent lack of genes coding for sphingolipid biosynthesis enzymes in theB. bacteriovorusgenome database. The results are consistent with the separation ofBdellovibrioandBacteriovorusinto separate genera.