Protective effects of ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1 on lipopolysaccharide-induced microcirculatory disturbance in rat mesentery
Protective effects of ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1 on lipopolysaccharide-induced microcirculatory disturbance in rat mesentery
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DOI：
10.1016/j.lfs.2007.06.008
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发表时间：
2007-07-19
期刊：
影响因子：
6.1
通讯作者：
Han, Jing-Yan
中科院分区：
文献类型：
--
作者：
Sun, Kai;Wang, Chuan-She;Han, Jing-Yan
人参皂苷Rb1(Rb1)。人参皂苷Rg1(Rg1)和三七皂苷R1(R1)是三七的主要活性成分，三七是一种广泛用于活血化瘀的中草药。采用倒置显微镜和高速摄像系统连续观察给药过程中大鼠肠系膜血管血流动力学变化，以评价其对脂多糖(LPS)所致微循环障碍的影响。给予LPS可降低红细胞流速，但Rb1、Rg1和RI可减弱这种影响。脂多糖可引起白细胞与静脉壁的黏附、肥大细胞脱颗粒和细胞因子的释放。Rb1、Rg1和R1减少贴壁白细胞数，抑制肥大细胞脱颗粒和细胞因子升高。流式细胞仪的体外实验进一步证实：1)Rb1和R1可显著抑制中性粒细胞表达CD11b/CD18；2)Rg1和R1可抑制中性粒细胞对内毒素刺激释放过氧化氢(H_2O_2)。提示Rb1和RI对脂多糖诱导的白细胞黏附的保护作用可能与其抑制中性粒细胞CD11b/CD18的表达有关。Rb1和R1对肥大细胞脱颗粒的保护作用，以及Rg1和R1钝化中性粒细胞释放H_2O_2的作用，提示三七总皂甙在减轻脂多糖诱导的微循环障碍中的作用机制不同。(C)2007年，爱思唯尔公司出版。
Ginsenoside Rb1 (Rb1). ginsenoside Rg1 (Rg1), and notoginsenoside R1 (R1) are major active components of Panalc notoginseng, a Chinese herb that is widely used in traditional Chinese medicine to enhance blood circulation and dissipate blood stasis. To evaluate the effect of these saponins on microcirculatory disturbance induced by lipopolysaccharide (LPS), vascular hemodynamics in rat mesentery was observed continuously during their administration using an inverted microscope and a high speed video camera system. LPS administration decreased red blood cell velocity but Rb1, Rg1, and RI attenuated this effect. LPS administration caused leukocyte adhesion to the venular wall, mast cell degranulation, and the release of cytokines. Rb1, Rg1, and R1 reduced the number of adherent leukocytes, and inhibited mast cell degranulation and cytokine elevation. In vitro experiments using flow cytometry further demonstrated that a) the LPS-enhanced expression of CD11b/CD 18 by neutrophils was significantly depressed by Rb1 and R 1, and b) hydrogen peroxide (H2O2) release from neutrophils in response to LPS stimulation was inhibited by treatment with Rg1 and R1. These results suggest that the protective effect of Rb1 and RI against leukocyte adhesion elicited by LPS may be associated with their suppressive action on the expression of CD11b/CD 18 by neutrophils. The protective effect against mast cell degranulation by Rb1 and R1, and the blunting of H2O2 release from neutrophils by Rg1 and R1 suggest mechanistic diversity in the effects of Panax notoarnseng saponins in the attenuation of microcirculatory disturbance induced by LPS. (c) 2007 Published by Elsevier Inc.