Rhabdomere turnover and rhodopsin cycle: maintenance of retinula cells inDrosophila melanogaster
Rhabdomere turnover and rhodopsin cycle: maintenance of retinula cells inDrosophila melanogaster
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横纹粒周转和视紫红质循环：果蝇视网膜细胞的维持
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
Donna Schilly
中科院分区：
文献类型：
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作者：
W. S. Stark;R. Sapp;Donna Schilly
果蝇视觉感受器的维持涉及膜的更新。自噬早期的特征是由横纹肌和质膜形成的被膜小凹、被膜小泡和直径约0.5 μm的多泡体组成。较小的电子致密体（0.15 μm）与MVB合并。这些很可能是初级溶酶体，如正常果蝇和酸性磷酸酶缺陷突变体中酸性磷酸酶组织化学所示。细胞外膜的聚集体证实了早期的报道，即胞吐脱落也可以在具有开放的横纹肌的果蝇中使用。显微分光光度法用于确定视色素循环的各个方面，以开始与已知的超微结构水平上的膜循环相关。视觉色素在黎明后3小时减少到一半，然后逐渐恢复到黎明前的最大值。我们对组织的固定是在黎明后时期，从视觉色素水平推断，自噬可能很高。在试图优化我们的营业额过程的建设性阶段的可视化，我们开发了类胡萝卜素的“替代疗法”的范例。类胡萝卜素替代的果蝇表现出视觉色素的增加，可能与膜流入横纹肌有关。老年苍蝇，研究以确定如何有效的维护，有一个独特的积累细胞外碎片，和一小部分的受体最终退化。
SummaryVisual receptor maintenance inDrosophila involves turnover of membrane. Coated pits derived from rhabdomere and plasmalemma, coated vesicles and multivesicular bodies (MVBs) of about 0.5 μm diameter characterize the early autophagic steps. Smaller electron dense bodies (0.15 μm) merge with MVBs. These are likely to be primary lysosomes as suggested by histochemistry for acid phosphatase in normal flies and an acid phosphatase deficient mutant. Aggregates of extracellular membranes confirm an earlier report that exocytotic shedding may also be employed in the fly with its open rhabdomeres. Microspectrophotometry was used to determine aspects of cycling of visual pigment to begin to correlate with what is known about membrane cycling at the ultrastuctural level. Visual pigment decreases to about half 3 h after dawn then builds back gradually to maximum before dawn. Our fixations of tissue have been at the post-dawn period when autophagy may be high as inferred from visual pigment levels. In attempts to optimize our visualization of the constructive phase of the turnover process, we developed the paradigm of carotenoid ‘replacement therapy’. Carotenoid replaced flies show an increase in visual pigment possibly associated with a streaming of membrane into the rhabdomere. Aged flies, studied to determine how effective maintenance is, have a unique accumulation of extracellular debris, and a small fraction of the receptors eventually degenerate.
影响因子：
4.4
作者：
Bok,D
通讯作者：
Bok,D
衣霉素诱导的视网膜杆外节膜发育不全。
影响因子：
4.4
作者：
Defoe,DM;Besharse,JC;Fliesler,SJ
通讯作者：
Fliesler,SJ