Photoperiod controls insulin and juvenile hormone signaling pathways via the circadian clock in the bean bug Riptortus pedestris (Hemiptera: Alydidae)
Photoperiod controls insulin and juvenile hormone signaling pathways via the circadian clock in the bean bug Riptortus pedestris (Hemiptera: Alydidae)
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DOI：
10.1007/s13355-022-00795-5
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发表时间：
2022-08
影响因子：
1.3
通讯作者：
G. Mano;S. Goto
中科院分区：
文献类型：
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作者：
G. Mano;S. Goto
温带的大多数多化性昆虫进入滞育是对短日照的反应。光周期在这些生物体中通过光周期时间测量系统进行评估，该系统涉及昼夜节律钟，并激活或灭活内分泌器官或细胞以改变其生理状态。虽然昆虫光周期性的生理机制已被广泛研究，但生物钟和内分泌信号通路之间的分子联系仍不清楚。本研究对豆蝽Riptortus pedestris（F.） （半翅目：Alydidae），进入成虫（生殖）滞育的反应短的日子。长日照条件下，脑内表达的胰岛素样肽ILP 1基因表达上调，与生殖力有关; ILP 1基因的功能与保幼激素信号调控的光周期反应无关; Cyp 15基因表达上调，与卵巢发育有关，Cyp 15基因编码的环氧酶对保幼激素的合成至关重要。针对生物钟基因的RNA干扰消除了Ilp 1和Cyp 15的抑制，使雌性即使在滞育诱导的短日照下也能繁殖。因此，生物钟可能通过改变两个独立的内分泌途径中关键元素的表达来控制光周期反应。
Most multivoltine insects in temperate zones enter diapause in response to short days. The photoperiod is evaluated in these organisms by a photoperiodic time measurement system, which involves the circadian clock, and activates or inactivates endocrine organs or cells to alter their physiological status. Although the physiological mechanisms underlying insect photoperiodism have been extensively studied, the molecular linkage between the circadian clock and endocrine signaling pathways remains unclear. In this study, we evaluated the bean bugRiptortus pedestris(F.) (Hemiptera: Alydidae), which enters adult (reproductive) diapause in response to short days. A gene encoding the insulin-like peptide ILP1, which is expressed in the pars intercerebralis in the brain, was upregulated and involved in fecundity under long days.Ilp1appeared to function independently of the photoperiodic response controlled by juvenile hormone signaling.Cyp15, which encodes an epoxidase crucial for juvenile hormone biosynthesis, was upregulated and involved in ovarian development under long days. RNA interference targeted against the circadian clock genepercanceled theIlp1andCyp15suppression and allowed females to be reproductive even under diapause-inducing short days. Thus, the circadian clock may control the photoperiodic response by altering the expression of key elements in two independent endocrine pathways.