bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice
bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice
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bZIP转录因子OsbZIP52/RISBZ5：水稻寒冷和干旱胁迫反应的潜在负调节因子
DOI：
10.1007/s00425-011-1564-z
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发表时间：
2012-06-01
期刊：
影响因子：
4.3
通讯作者：
Wang, Xiping
中科院分区：
文献类型：
--
作者：
Liu, Citao;Wu, Yanbin;Wang, Xiping
OsbZIP 52/RISBZ 5是水稻（Oryza sativa）碱性亮氨酸拉链（bZIP）转录因子家族的成员之一。OsbZIP 52基因的表达强烈诱导低温（4A摄氏度），但不是由干旱，PEG，盐，或阿坝。OsbZIP 52-GFP在洋葱表皮细胞中的亚细胞定位表明OsbZIP 52是一个核定位蛋白。酵母中的反式激活试验证明OsbZIP 52作为转录激活因子发挥功能，并且可以特异性地结合到G-box启动子基序。在酵母双杂交（Y-2-H）实验中，OsbZIP 52能够形成同源二聚体复合物。过量表达OsbZIP 52的水稻植株表现出对寒冷和干旱胁迫的敏感性显著增加。实时荧光定量PCR分析表明，OsLEA 3、OsTPP 1、Rab 25、gp 1前体、beta-gal、LOC_Os05g11910和LOC_Os05g39250等非生物胁迫相关基因在OsbZIP 52过表达系中表达下调。这些结果表明OsbZIP 52/RISBZ 5在寒冷和干旱胁迫环境中可能起负调节作用。
OsbZIP52/RISBZ5 is a member of the basic leucine zipper (bZIP) transcription factor (TF) family in rice (Oryza sativa) isolated from rice (Zhonghua11) panicles. Expression of the OsbZIP52 gene was strongly induced by low temperature (4A degrees C) but not by drought, PEG, salt, or ABA. The subcellular localization of OsbZIP52-GFP in onion (Allium cepa) epidermis cells revealed that OsbZIP52 is a nuclear localized protein. A transactivation assay in yeast demonstrated that OsbZIP52 functions as a transcriptional activator and can specifically bind to the G-box promoter motif. In a yeast two-hybrid (Y-2-H) experiment, OsbZIP52 was able to form homodimeric complexes. Rice plants overexpressing OsbZIP52 showed significantly increased sensitivity to cold and drought stress. Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, beta-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines. These results suggest that OsbZIP52/RISBZ5 could function as a negative regulator in cold and drought stress environments.