Aggregatibacter actinomycetemcomitans adhesion inhibited in a flow cell
Aggregatibacter actinomycetemcomitans adhesion inhibited in a flow cell
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DOI：
10.1111/j.1399-302x.2008.00456.x
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发表时间：
2008-12-01
影响因子：
--
通讯作者：
Teughels, W.
中科院分区：
文献类型：
--
作者：
Sliepen, I.;Hofkens, J.;Teughels, W.
微生物相互作用在致病菌在口腔内的粘附过程中被认为是重要的。本研究提出了一种假设，即在流体动力学条件下，链球菌生物膜影响牙周病原聚集菌放线菌在硬组织中的定植。方法：利用安装在共聚焦激光扫描显微镜上的流动细胞，实时监测绿色荧光蛋白标记放线菌在涂有链球菌生物膜表面的定植情况。培养和定量聚合酶链反应数据由改良罗宾斯装置并行获得。结果：四种被试链球菌（血链球菌、cristatus链球菌、唾液链球菌和mitis链球菌）均能抑制放线菌的定植。抑制作用最强的是血螺。结论：这些结果证实了在水动力条件下，某些细菌种类会影响放线菌在体外坚硬表面的定殖。
Introduction: Microbial interactions are considered important in the adhesion process of pathogenic bacteria in the oral cavity. This study addressed the hypothesis that a streptococcal biofilm influences the hard tissue colonization by the periodontopathogen Aggregatibacter actinomycetemcomitans under hydrodynamic conditions.Methods: The colonization of a green-fluorescent-protein-labelled A. actinomycetemcomitans strain on surfaces coated with a streptococcal biofilm, was monitored in real time using a confocal laser scanning microscope-mounted flow cell. Culture and quantitative polymerase chain reaction data were obtained in parallel from a Modified Robbins Device.Results: Colonization of A. actinomycetemcomitans was inhibited by the four tested streptococci (Streptococcus sanguinis, Streptococcus cristatus, Streptococcus salivarius, and Streptococcus mitis). The most inhibiting species was S. sanguinis.Conclusions: These results confirmed the hypothesis that some bacterial species influence A. actinomycetemcomitans colonization of hard surfaces in vitro under hydrodynamic conditions.