Rad9 plays an important role in DNA mismatch repair through physical interaction with MLH1.
Rad9 plays an important role in DNA mismatch repair through physical interaction with MLH1.
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Rad9 通过与 MLH1 的物理相互作用在 DNA 错配修复中发挥重要作用
DOI：
10.1093/nar/gkn686
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发表时间：
2008-11
影响因子：
14.9
通讯作者：
Hang H
中科院分区：
文献类型：
--
作者：
He W;Zhao Y;Zhang C;An L;Hu Z;Liu Y;Han L;Bi L;Xie Z;Xue P;Yang F;Hang H
Rad 9是从酵母到人类的保守基因，在DNA修复（同源重组修复和碱基对切除修复）和细胞周期检查点控制中发挥作用。Rad 9是否参与DNA错配修复（MMR）尚未见报道。在这项研究中，我们已经证明，人类和小鼠Rad 9与MMR蛋白MLH 1物理相互作用。通过Rad 9中的单点突变破坏相互作用导致MMR活性显著降低。这种破坏不影响S/M检查点控制和第一轮G2/M检查点控制，也不改变细胞对UV光、γ射线或羟基脲的敏感性。我们的数据表明，Rad 9是MMR中的一个重要因子，并通过与MLH 1的相互作用特异性地执行其MMR功能。
Rad9 is conserved from yeast to humans and plays roles in DNA repair (homologous recombination repair, and base-pair excision repair) and cell cycle checkpoint controls. It has not previously been reported whether Rad9 is involved in DNA mismatch repair (MMR). In this study, we have demonstrated that both human and mouse Rad9 interacts physically with the MMR protein MLH1. Disruption of the interaction by a single-point mutation in Rad9 leads to significantly reduced MMR activity. This disruption does not affect S/M checkpoint control and the first round of G2/M checkpoint control, nor does it alter cell sensitivity to UV light, gamma rays or hydroxyurea. Our data indicate that Rad9 is an important factor in MMR and carries out its MMR function specifically through interaction with MLH1.