Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm.
Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm.
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DOI：
10.1155/2012/127149
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发表时间：
2012
影响因子：
1.3
通讯作者：
Yamagishi M
中科院分区：
文献类型：
--
作者：
Sakamoto A;Kawashiri M;Ishibashi-Ueda H;Sugamoto Y;Yoshimuta T;Higashikata T;Ogino H;Tada H;Konno T;Hayashi K;Yamagishi M
我们研究了ephrin-B1及其同源受体EphB 2的表达，血管生成和胚胎发生的关键调节因子，在人类腹主动脉瘤（AAA），并分析其在细胞迁移的功能作用。我们从10名因AAA接受血管手术的患者（9名男性和1名女性;年龄，68.5 ± 2.4）中获得AAA和邻近对照组织。使用实时RT-PCR，我们分析了ephrin-B1和EphB 2的表达。我们还在AAA组织中定位了这些分子。最后，通过细胞迁移试验检测ephrin-B1和EphB 2对炎性细胞趋化性的影响。Ephrin-B1（0.410 ± 0.046vs1.198 ± 0.252，P = 0.027）和EphB 2（0.764 ± 0.212vs1.272 ± 0.137，P = 0.594）在AAA中的表达水平高于正常对照组。ephrin-B1和EphB 2均在AAA内的巨噬细胞、T淋巴细胞和内皮细胞中表达。在趋化实验中，ephrin-B1和EphB 2对基质衍生因子-1诱导的单核细胞趋化的抑制率分别为54.7 ± 12.7%（P = 0.01）和50.7 ± 13.1%（P = 0.01）。这些数据表明，ephrin-B1和EphB 2可能在人类成人炎症细胞中发挥作用，并参与AAA的发病机制，但这些分子的具体作用还有待进一步研究。
We examined the expression of ephrin-B1 and its cognate receptor EphB2, key regulators of angiogenesis and embryogenesis, in human abdominal aortic aneurysm (AAA) and analyzed their functional roles in cell migration. From 10 patients (9 males and 1 female; age, 68.5 ± 2.4) who underwent vascular surgery for AAA, we obtained AAA and adjacent control tissues. Using real-time RT-PCR, we analyzed expression of ephrin-B1 and EphB2. We also histologically localized these molecules in AAA tissues. Finally, effects of ephrin-B1 and EphB2 on inflammatory cell chemotaxis were examined by cell migration assay. Expression levels of ephrin-B1 (0.410 ± 0.046 versus 1.198 ± 0.252, P = 0.027) and EphB2 (0.764 ± 0.212 versus 1.272 ± 0.137, P = 0.594) were higher in AAA than normal control. Both ephrin-B1 and EphB2 were expressed in macrophages, T lymphocytes, and endothelial cells within AAA. In chemotaxis assay, ephrin-B1 and EphB2 inhibited mononuclear-cell chemotaxis induced by stromal derived factor-1 down to 54.7 ± 12.7% (P = 0.01) and 50.7 ± 13.1% (P = 0.01), respectively. These data suggest that ephrin-B1 and EphB2 might be functional in human adult inflammatory cells and involved in the pathogenesis of AAA, although specific roles of these molecules should further be sought.