A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
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捕食性粘细菌通过调节土壤微生物群落控制黄瓜枯萎病
DOI：
10.1186/s40168-020-00824-x
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发表时间：
2020-04
期刊：
影响因子：
15.5
通讯作者：
Ruifu Zhang
中科院分区：
文献类型：
--
作者：
Xianfeng Ye;Zhoukun Li;Xue Luo;Wenhui Wang;Yongkai Li;Rui Li;Bo Zhang;Yan Qiao;Jie Zhou;Jiaqin Fan;Hui Wang;Yan Huang;Hui Cao;Zhongli Cui;Ruifu Zhang
背景粘细菌是土壤生态系统中的微型捕食者，具有协同活动和取食的能力。一些粘细菌菌株已被用于控制土传真菌植物病原菌。然而，粘性细菌、植物病原菌和土壤微生物之间的相互作用在很大程度上还没有被探索。在这项研究中，我们的目的是调查粘性细菌珊瑚球藻的行为。黄瓜枯萎病防治接种后土壤中的EGb菌株及其对土壤微生物群的影响结果温室和2年田间试验表明，固态发酵菌株EGB对黄瓜枯萎病的防效分别为79.6%(温室)、66.0%(2015田间)和53.9%(2016田间)。菌株EGb对土壤环境适应性强，能有效降低土壤中FOC的丰度。土壤微生物群落的时空分析表明，菌株EGb通过趋化作用向黄瓜植株的根部和根系分泌物迁移。土壤微生物组的共生网络分析表明，施加菌株EGB后，土壤微生物群落的模块化程度和群落数量降低，但每节的连接数增加。溶杆菌、微病毒杆菌和铜绿假单胞菌等几种捕食性细菌以枢纽或指示物的形式出现，与其他细菌有密切的联系。EGb菌株通过向植物根部迁移和调节土壤微生物群落来控制黄瓜枯萎病。该菌株有可能被开发为土传枯萎病的新型生防剂。
BackgroundMyxobacteria are micropredators in the soil ecosystem with the capacity to move and feed cooperatively. Some myxobacterial strains have been used to control soil-borne fungal phytopathogens. However, interactions among myxobacteria, plant pathogens, and the soil microbiome are largely unexplored. In this study, we aimed to investigate the behaviors of the myxobacteriumCorallococcussp. strain EGB in the soil and its effect on the soil microbiome after inoculation for controlling cucumber Fusarium wilt caused byFusarium oxysporumf. sp.cucumerinum(FOC).ResultsA greenhouse and a 2-year field experiment demonstrated that the solid-state fermented strain EGB significantly reduced the cucumber Fusarium wilt by 79.6% (greenhouse), 66.0% (2015, field), and 53.9% (2016, field). Strain EGB adapted to the soil environment well and decreased the abundance of soil-borne FOC efficiently. Spatiotemporal analysis of the soil microbial community showed that strain EGB migrated towards the roots and root exudates of the cucumber plants via chemotaxis. Cooccurrence network analysis of the soil microbiome indicated a decreased modularity and community number but an increased connection number per node after the application of strain EGB. Several predatory bacteria, such asLysobacter,Microvirga, andCupriavidus, appearing as hubs or indicators, showed intensive connections with other bacteria.ConclusionThe predatory myxobacteriumCorallococcussp. strain EGB controlled cucumber Fusarium wilt by migrating to the plant root and regulating the soil microbial community. This strain has the potential to be developed as a novel biological control agent of soil-borne Fusarium wilt.Video abstract.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
11
作者：
Pasternak, Zohar;Pietrokovski, Shmuel;Jurkevitch, Edouard
通讯作者：
Jurkevitch, Edouard
土壤原生生物群落形成土壤微生物组的动态中心
DOI：
10.1038/ismej.2017.171
发表时间：
2017-10
期刊：
The ISME Journal
影响因子：
--
作者：
Wu Xiong;Alex;re Jousset;Sai Guo;Ida Karlsson;Qingyun Zhao;Huasong Wu;George A Kowalchuk;Qirong Shen;Rong Li;Stefan Geisen
通讯作者：
Stefan Geisen
影响因子：
4.8
作者：
Pu Yang;J. D. Elsas
通讯作者：
Pu Yang;J. D. Elsas
影响因子：
11.3
作者：
Berleman JE;Kirby JR
通讯作者：
Kirby JR