Glia atrophy in the hippocampus of chronic unpredictable stress-induced depression model rats is reversed by electroacupuncture treatment
Glia atrophy in the hippocampus of chronic unpredictable stress-induced depression model rats is reversed by electroacupuncture treatment
复制标题
电针治疗可逆转慢性不可预测应激抑郁模型大鼠海马胶质细胞萎缩
DOI：
10.1016/j.jad.2010.07.007
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发表时间：
2011-02-01
影响因子：
6.6
通讯作者：
Wu, Gen-Cheng
中科院分区：
文献类型：
--
作者：
Liu, Qiong;Li, Bing;Wu, Gen-Cheng
背景资料：越来越多的证据表明，神经胶质萎缩有助于病理生理和可能的发病机制的抑郁症。电针是我国传统的治疗方法之一，临床研究显示其具有抗抑郁样作用。方法：采用慢性不可预见性应激（CUS）所致抑郁模型大鼠，观察电针对抑郁症的治疗作用。电针治疗组于CUS术后2周电针“百会”穴和单侧“暗面”穴，每日1次，连续3周。采用物理状态（PS）和旷场实验（OFT）分析电针治疗的抗抑郁样作用。采用免疫组化、Western blot和RT-PCR方法检测海马星形胶质细胞标志物胶质细胞酸性蛋白（GFAP）的表达。抑郁模型大鼠海马GFAP mRNA和蛋白表达水平降低。有趣的是，电针治疗有效地阻断了GFAP水平的下降。限制：结论：电针对抑郁模型大鼠具有潜在的抗抑郁样作用，其机制可能是通过影响海马神经胶质细胞的萎缩而实现的。(C)2010 Elsevier B. V.保留所有权利。
Background: Growing evidence indicates that glia atrophy contributes to the pathophysiology and possibly the pathogenesis of major depressive disorder. Electroacupuncture (EA), one of Chinese traditional therapy, has potent antidepressant-like effect in many clinical studies. The mechanism by which EA improves behavioral deficits is still unclear.Method: Chronic unpredictable stress (CUS)-induced depression model rats were used to study the effect of EA treatment. EA was performed on acupoints 'Bai-Hui' (Du 20) and unilateral 'An-Mian' (EX 17) once daily for three consecutive weeks two weeks post CUS procedure. The antidepressant-like effect of EA treatment was analyzed by physical state (PS) and open field test (OFT). Astrocytic marker glial fibrillary acidic protein (GFAP) level in the hippocampus was detected by immunohistochemsitry, Western blot analysis and reverse transcription-polymerase chain reaction (RT-PCR).Results: Exposure to CUS resulted in a decrease of behavioral activity, whereas a daily session of EA treatment significantly reversed the behavioral deficit of these depression model rats. Moreover, the levels of GFAP mRNA and protein were decreased in the hippocampus of depression model rats. Intriguingly, EA treatment blocked effectively the decreased GFAP level. Limitation: The relative small number of the depression model rats may cause some bias of behavioral tests.Conclusion: EA has potential antidepressant-like effect on CUS-induced depression model rats, which might be mediated by affecting the glial atrophy in the hippocampus. (C) 2010 Elsevier B.V. All rights reserved.