Indole-based alkaloids from deep-sea bacterium Shewanella piezotolerans with antitumor activities
Indole-based alkaloids from deep-sea bacterium Shewanella piezotolerans with antitumor activities
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DOI：
10.1038/ja.2014.3
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发表时间：
2014-05-01
影响因子：
3.3
通讯作者：
Lin, Wenhan
中科院分区：
文献类型：
--
作者：
Wang, Yao;Tang, Xixiang;Lin, Wenhan
从深海采集的化学未知细菌Shewanella piezotolerans WP 3的发酵液中获得的粗提物进行色谱分离，产生了三种新的吲哚生物碱，即Shewanellines A（1a）、B（1 B）和C（2），以及12种已知的吲哚生物碱。根据1D和2D NMR（H-1、C-13、COSY、HMBC、HSQC和NOESY）结合MS和CD数据明确阐明了结构。选择化合物1-4、7、9和11- 1 - 4用于评价它们对人肿瘤细胞系HL-60和BEL-7402的细胞毒活性，而化合物2、4和9对HL-60表现出显著的抑制作用。
Chromatographic separation of a crude extract obtained from a fermentation broth of a chemically unknown bacterium Shewanella piezotolerans WP3 collected in deep-sea yielded three new indole alkaloids namely shewanellines A (1a), B (1b) and C (2), together with 12 known indole alkaloids. The structures were unambiguously elucidated on the basis of 1D and 2D NMR (H-1, C-13, COSY, HMBC, HSQC and NOESY) in association with MS and CD data. Compounds 1-4, 7, 9 and 11-14 were selected for the evaluation of their cytotoxic activities against human tumor cell lines HL-60 and BEL-7402, whereas compounds 2, 4 and 9 exhibited significant inhibition toward HL-60.