Response of rainbow trout transcriptome to model chemical contaminants
Response of rainbow trout transcriptome to model chemical contaminants
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DOI：
10.1016/j.bbrc.2004.06.024
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发表时间：
2004-07-30
影响因子：
3.1
通讯作者：
Oikari, A
中科院分区：
文献类型：
--
作者：
Koskinen, H;Pehkonen, P;Oikari, A
我们使用高密度基因芯片研究了虹鱼鱼苗对β-萘黄酮、镉、四氯化碳和芘的亚致死范围的反应。根据基因本体论的功能分类，对差异表达基因进行了分类。在细胞周期、细胞凋亡、信号转导、氧化应激、亚细胞和胞外结构、蛋白质生物合成和修饰等方面，对所研究化合物的反应明显不同。聚类分析分离了对低剂量和中等剂量污染物的反应，而在高剂量时，适应性反应被一般的非特异性毒性反应所掩盖。我们发现许多线粒体蛋白以及参与金属离子代谢和蛋白质生物合成的基因表达增强。同时，与应激和免疫反应、信号转导和核苷酸代谢相关的基因下调。我们对检索到的每种化合物的Medline摘要进行了计算机辅助分析，这有助于我们指出预期的和新的发现。(C)2004 Elsevier Inc.保留所有权利。
We used high-density cDNA microarray in studies of responses of rainbow trout fry at sublethal ranges of beta-naphthoflavone, cadmium, carbon tetrachloride, and pyrene. The differentially expressed genes were grouped by the functional categories of Gene Ontology. Significantly different response to the studied compounds was shown by a number of classes, such as cell cycle, apoptosis, signal transduction, oxidative stress, subcellular and extracellular structures, protein biosynthesis, and modification. Cluster analysis separated responses to the contaminants at low and medium doses, whereas at high levels the adaptive reactions were masked with general unspecific response to toxicity. We found enhanced expression of many mitochondrial proteins as well as genes involved in metabolism of metal ions and protein biosynthesis. In parallel, genes related to stress and immune response, signal transduction, and nucleotide metabolism were down-regulated. We performed computer-assisted analyses of Medline abstracts retrieved for each compound, which helped us to indicate the expected and novel findings. (C) 2004 Elsevier Inc. All rights reserved.