EFFECTS OF DELETIONS IN THE UNCA-UNCG INTERGENIC REGIONS ON EXPRESSION OF UNCG, THE GENE FOR THE GAMMA-SUBUNIT OF THE ESCHERICHIA-COLI F1F0-ATPASE
EFFECTS OF DELETIONS IN THE UNCA-UNCG INTERGENIC REGIONS ON EXPRESSION OF UNCG, THE GENE FOR THE GAMMA-SUBUNIT OF THE ESCHERICHIA-COLI F1F0-ATPASE
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DOI：
10.1016/0005-2728(94)90077-9
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发表时间：
1994-01-04
影响因子：
4.3
通讯作者：
BRUSILOW, WSA
中科院分区：
文献类型：
--
作者：
ANGOV, E;BRUSILOW, WSA
E. coliF 1F 0-ATPase由uncG编码。与unc操纵子中位于uncG之前的uncA（ct亚基）相比，该基因表达较差。这些基因由50个核苷酸的基因间区域分开。我们研究了该区域中的一组缺失对位于染色体unc操纵子或染色体λ att位点的uncA“-”lacZ和uncG“-”lacZ翻译融合基因的相对表达的影响。根据染色体位置，α亚基基因的表达比γ亚基基因好3-6倍。缺失分析表明，uncA-uncG基因间区域显着影响uncG的表达，但Shine-Dalgarno区域是不是uncG的表达绝对必需的。不同的缺失导致uncG的表达增加或减少。
The gamma subunit of the E. coli F1F0-ATPase is coded for by uncG. This gene is poorly expressed compared to uncA (ct subunit), which precedes uncG in the unc operon. The genes are separated by a 50-nucleotide intergenic region. We examined the effects of a set of deletions in this region on the relative expression of uncA'-'lacZ and uncG'-'lacZ translational fusion genes located either in the chromosomal unc operon or at the chromosomal lambda att site. The gene for the a subunit was expressed 3-6-times better than the gene for the gamma subunit, depending upon chromosomal location. Deletion analysis revealed that the uncA-uncG intergenic region significantly affects expression of uncG, but the Shine-Dalgarno region is not absolutely required for expression of uncG. Different deletions resulted in either increased or decreased expression of uncG.