Glypican-3 expression in Wilms tumor and hepatoblastoma
Glypican-3 expression in Wilms tumor and hepatoblastoma
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DOI：
10.1097/00043426-200111000-00006
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发表时间：
2001-11-01
影响因子：
1.2
通讯作者：
Schlessinger, D
中科院分区：
文献类型：
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作者：
Toretsky, JA;Zitomersky, NL;Schlessinger, D
背景：Glypican-3 (GPC3) 是一种硫酸乙酰肝素蛋白聚糖。当它被破坏时，就会导致 X 连锁巨人症过度生长辛普森-戈拉比-贝梅尔综合征。它参与生长控制与最近的报道一致，即它可以与生长因子结合，可能包括胰岛素样生长因子 2。此外，据推测它可能在乳腺癌、卵巢癌和间皮瘤中充当肿瘤抑制基因。患者和方法：从肾母细胞瘤和肝母细胞瘤组织样本中提取 RNA 和蛋白质，并通过 Northern 印迹、逆转录聚合酶链反应和免疫印迹测量这些提取物中的 GPC3 水平。结果：与已发表的结果相反癌症研究结果显示，在肾母细胞瘤和肝母细胞瘤中发现高水平的 GPC3 表达。在肿瘤周围的正常组织中发现该基因的表达较低或检测不到。结论：肾母细胞瘤和肝母细胞瘤中 GPC3 表达增加表明该基因产物具有生长促进或中性活性，而不是生长抑制作用。
Background: Glypican-3 (GPC3) is a heparan sulfate proteoglycan. When it is disrupted, it causes the X-linked gigantism-overgrowth Simpson-Golabi-Behmel syndrome. Its involvement in growth control is consistent with recent reports that it can bind to growth factors, possibly including insulin-like growth factor 2. Further, it has been hypothesized that it may function as a tumor suppressor gene in breast and ovarian carcinomas and mesotheliomas.Patients and Methods: RNA and protein were extracted from Wilms tumor and hepatoblastoma tissue samples and GPC3 levels were measured in these extracts by Northern blotting, reverse transcription polymerase chain reaction, and immunoblotting.Results: In contrast to published results with carcinomas, high levels of GPC3 expression were found in Wilms tumor and hepatoblastoma. Low or undetectable expressions of this gene were found in normal tissue surrounding the tumor.Conclusions: Increased expression of GPC3 in Wilms tumor and hepatoblastoma suggests a growth-promoting or neutral activity for this gene product rather than a growth-suppressive effect.