Novel methyltransferase for modified uridine residues at the wobble position of tRNA
Novel methyltransferase for modified uridine residues at the wobble position of tRNA
复制标题
DOI：
10.1128/mcb.23.24.9283-9292.2003
复制
发表时间：
2003-12-01
影响因子：
5.3
通讯作者：
Clarke, S
中科院分区：
文献类型：
--
作者：
Kalhor, HR;Clarke, S
我们在酿酒酵母中发现了一种新的tRNA甲基转移酶，我们将其命名为Trm9。该酶是YML014w基因的产物，催化修饰的尿嘧啶核苷酸的酯化反应，导致tRNA中的5-甲基羰基甲基尿嘧啶（Arg3）和tRNA中的5-甲基羰基甲基-2-硫脲（Glu）形成。在完整的酵母细胞中，TRM9基因的破坏导致这些修饰的摆动碱基完全丧失，并且对paromomycin（一种翻译抑制剂）的敏感性增加37度。这些结果表明，当细胞处于应激状态时，这种潜在可逆的甲基酯化反应的作用。
We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37degreesC to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.