Porous gelatin-chondroitin-hyaluronate tri-copolymer scaffold containing microspheres loaded with TGF-β1 induces differentiation of mesenchymal stem cells in vivo for enhancing cartilage repair
Porous gelatin-chondroitin-hyaluronate tri-copolymer scaffold containing microspheres loaded with TGF-β1 induces differentiation of mesenchymal stem cells in vivo for enhancing cartilage repair
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DOI：
10.1002/jbm.a.30647
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发表时间：
2006-06-15
影响因子：
4.9
通讯作者：
Lv, Rong
中科院分区：
文献类型：
--
作者：
Fan, Hongbin;Hu, Yunyu;Lv, Rong
该研究的目的是生产一种新型的多孔明胶-软骨素-透明质酸盐支架，结合转化生长因子β 1（TGF-β 1）的受控释放，诱导体内间充质干细胞（MSC）的分化，以增强软骨修复。载TGF-β 1的明胶微球（MSTGF β 1）在初始阶段显示快速释放（37.4%），并且到第18天，最终累积释放为83.1%。将自体骨髓间充质干细胞接种于MS-TGF β 1/支架材料上修复兔全层软骨缺损作为体内分化修复组，将体外分化的骨髓间充质干细胞接种于不含MS-TGF β 1的支架材料上修复对侧软骨缺损（n = 30）。另外15只未接受缺损治疗的家兔用作对照。组织学观察显示，术后12周和24周，体内分化修复组软骨细胞形态较体外分化修复组好，软骨下骨连续，新生软骨层较体外分化修复组厚。两组间组织学分级评分差异有统计学意义，均显示修复效果明显优于对照组。本研究提示，含MS-TGF β 1的新型支架材料可能成为体内诱导MSCs分化促进软骨修复的新途径。(c)2006 Wiley Periodicals，Inc.
The aim of the study was to produce a novel porous gelatin-chondroitin-hyaluronate scaffold in combination with a controlled release of transforming growth 0 factor beta 1 (TGF-beta 1), which induced the differentiation of mesenchymal stem cells (MSCs) in vivo for enhancing cartilage repair. Gelatin microspheres loaded with TGF-beta 1 (MSTGF beta 1) showed a fast release at the initial phase (37.4%,), and the ultimate accumulated release,vas 83.1%, by day 18. The autologous NMSCs seeded on MS-TGF beta 1/scaffold were implanted to repair full-thickness cartilage defects in rabbits as in vivo differentiation repair group, while MSCs differentiated in vitro were seeded on scaffold without MS-TGF beta 1 to repair the contra lateral cartilage defects (n = 30). Fifteen additional rabbits without treatment for defects were used as control. Histology observation showed that the in vivo differentiation repair group had better chondrocyte morphology, integration, continuous subchondral bone, and much thicker newly formed cartilage layer when compared to in vilro differentiation repair group 12 and 24 weeks, postoperatively. There was a significant difference in histological grading score between these two experimental groups, and both showed much better repair than that of the control. The present study implied that the novel scaffold with MS-TGF beta 1 might serve as a new way to induce the differentiation of MSCs in vivo to enhance the cartilage repair. (c) 2006 Wiley Periodicals, Inc.