Transcranial optogenetic stimulation for functional mapping of the motor cortex
Transcranial optogenetic stimulation for functional mapping of the motor cortex
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DOI：
10.1016/j.jneumeth.2009.02.001
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发表时间：
2009-05-15
影响因子：
3
通讯作者：
Matsuzaki, Masanori
中科院分区：
文献类型：
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作者：
Hira, Riichiro;Honkura, Naoki;Matsuzaki, Masanori
我们开发了一种使用视紫红质-2（ChR 2）进行经颅光遗传学刺激的方法。这种方法是基于扫描大脑的光束，从而光刺激完整小鼠中表达ChR 2的神经元。作为原理的证明，我们将这种技术应用于在皮质锥体细胞中表达ChR 2的转基因小鼠的运动皮质。光刺激诱导的肢体运动是以毫秒级精度锁定时间的，并且可以在高达20 Hz的频率下诱导。通过扫描这个光束，我们可以绘制出与肢体运动相关的神经元的分布。通过这种方法，我们可以同时定义控制两个肢体的运动地图，并可以在几周内重复生成这种皮层运动地图。这种方法允许对活体动物的脑回路进行非侵入性映射，并有助于确定行为和脑回路之间的联系。(C)2009 Elsevier B. V.保留所有权利。
We developed a method that uses Channelrhodopsin-2 (ChR2) for transcranial optogenetic stimulation. This method is based on scanning a light beam over the brain, thereby photostimulating ChR2-expressing neurons in intact mice. As a proof of principle, we applied this technique to the motor cortex of transgenic mice expressing ChR2 in cortical pyramidal cells. Photostimulation induced limb movements that were time-locked with millisecond precision and could be induced at frequencies up to 20 Hz. By scanning this light beam, we could map the distribution of neurons associated with limb movement. With this approach we could simultaneously define motor maps controlling two limbs and could reproducibly generate such cortical motor maps over periods of weeks. This method allows non-invasive mapping of brain circuitry in living animals and could help define the connection between behavior and brain circuitry. (C) 2009 Elsevier B.V. All rights reserved.