Cats are protected against feline immunodeficiency virus infection following vaccination with a homologous AP-1 binding site-deleted mutant
Cats are protected against feline immunodeficiency virus infection following vaccination with a homologous AP-1 binding site-deleted mutant
复制标题
猫在接种同源 AP-1 结合位点缺失突变体疫苗后可免受猫免疫缺陷病毒感染
作者：
M. Kohmoto;T. Miyazawa;E. Sato;K. Uetsuka;Y. Nishimura;Y. Ikeda;G. Inada;K. Doi;T. Mikami
猫免疫缺陷病毒（FIV）AP-1结合位点缺失突变体（ΔAP-1）经阴道途径感染猫后，用FIV同源完整株（TM 2）攻毒。观察猫23周，以评价ΔAP-1对同源TM 2菌株攻毒的有效性。用半巢式聚合酶链反应扩增外周血单个核细胞和组织中的前病毒DNA后，通过Southern印迹法区分这两种病毒。在1只接触ΔAP-1但未感染的猫中检测到TM 2特异性条带，但在2只ΔAP-1感染的猫中未检测到。这些结果表明，ΔAP-1可以保护免受同源FIV TM 2株的后续攻击。
SummaryFollowing establishment, via the vaginal route, of infection with an AP-1 binding-site deleted mutant (ΔAP-1) of feline immunodeficiency virus (FIV), cats were challenged with a homologous intact strain (TM2) of FIV. The cats were observed for 23 weeks to evaluate the efficacy of the ΔAP-1 against the homologous TM2 strain challenge. These two viruses were differentiated by Southern blotting after amplification of proviral DNA by semi-nested polymerase chain reaction in DNAs of peripheral blood mononuclear cells and tissues. A TM2-specific band was detected in one cat exposed to but not infected with ΔAP-1, but not in two ΔAP-1-infected. These results indicate that ΔAP-1 could protect against subsequent challenge with homologous FIV TM2 strain.
细胞相关和无细胞猫免疫缺陷病毒的粘膜传播。
DOI：
10.1089/aid.1997.13.347
发表时间：
1997
期刊：
AIDS research and human retroviruses.
影响因子：
--
作者：
Burkhard,MJ;Obert,LA;O'Neil,LL;Diehl,LJ;Hoover,EA
通讯作者：
Hoover,EA
影响因子：
1
作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen
通讯作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen