Experimental modeling of spinal cord injury: Characterization of a force-defined injury device
Experimental modeling of spinal cord injury: Characterization of a force-defined injury device
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DOI：
10.1089/08977150360547099
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发表时间：
2003-02-01
影响因子：
4.2
通讯作者：
Lumpp, JE
中科院分区：
文献类型：
--
作者：
Scheff, SW;Rabchevsky, AG;Lumpp, JE
我们研究了一种新型脊髓损伤（SCI）装置在成年大鼠胸10级（T10）损伤后产生分级形态和行为变化的能力。损伤器械使用施加到组织的力作为控制变量，而不是组织位移。这具有消除可能由损伤前的组织移动引起的误差的优点。在两个不同的生存时间（7和42天），三种不同的损伤严重程度，定义的力量施加到暴露的脊髓在T10（100，150和200 kdyn），进行了评价。采用无偏体视学评价损伤后的形态学差异。定量行为评估采用Basso、Beattie和Bresnahan机车评定量表。受伤后，运动能力出现明显的力相关性下降。受到200 kdyn损伤的动物的表现明显比受到100和150 kdyn损伤的动物差。伤后不同时间的运动能力与脊髓所受力的大小显著相关。统计分析显示，几个显着的力相关的形态学差异损伤后。白色和灰质的最大损失发生在损伤撞击部位，并向吻侧和尾侧方向延伸。动物受到最大的力（200 kdyn）显示最少量的备用组织在两个生存时间的指示最严重的损伤。备用组织的数量与运动能力显著相关。这种新的SCI啮齿动物模型通过用恒定的预设力来定义损伤来减少变异性，从而提供了对现有SCI装置的显著改进。
We examined the ability of a novel spinal cord injury (SCI) device to produce graded morphological and behavioral changes in the adult rat following an injury at thoracic level 10 (T10). The injury device uses force applied to the tissue as the control variable rather than tissue displacement. This has the advantage of eliminating errors that may arise from tissue movement prior to injury. Three different injury severities, defined by the amount of force applied to the exposed spinal cord at T10 (100, 150, and 200 kdyn), were evaluated at two different survival times (7 and 42 d). Unbiased stereology was employed to evaluate morphological differences following the injury. Quantitative behavioral assessment employed the Basso, Beattie, and Bresnahan locomotive rating scale. There was a significant force-related decline in locomotive ability following the injury. Animals subjected to a 200-kdyn injury performed significantly worse than animals subjected to a 100- and 150-kdyn injury. The locomotor ability at different days post injury significantly correlated with the amount of force applied to the spinal cord. Statistical analysis revealed several significant force-related morphological differences following the injury. The greatest loss of white and gray matter occurred at the site of injury impact and extended in both a rostral and caudal direction. Animals subjected to the greatest force (200 kdyn) displayed the least amount of spared tissue at both survival times indicative of the most severe injury. The amount of spared tissue significantly correlated with the locomotor ability. This novel rodent model of SCI provides a significant improvement over existing devices for SCI by reducing variability with a constant preset force to define the injury.