MicroRNA regulation in extreme environments: differential expression of microRNAs in the intertidal snail Littorina littorea during extended periods of freezing and anoxia.
MicroRNA regulation in extreme environments: differential expression of microRNAs in the intertidal snail Littorina littorea during extended periods of freezing and anoxia.
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DOI：
10.1016/j.gpb.2012.09.002
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发表时间：
2012-10
影响因子：
9.5
通讯作者：
Storey, Kenneth B.
中科院分区：
文献类型：
--
作者：
Biggar, Kyle K.;Kornfeld, Samantha F.;Maistrovski, Yulia;Storey, Kenneth B.
最近几项关于脊椎动物适应环境胁迫的研究表明，microRNA（miRNAs）在调节蛋白质合成和/或重组蛋白质表达模式的全局抑制中发挥作用。本研究是第一个表征应激反应的变化，在自然冷冻或缺氧暴露在无脊椎动物物种，潮间带腹足类Littorina littorea的miRNA的表达。这些蜗牛暴露在缺氧和寒冷的环境中，因为它们的环境不断地在潮汐和季节的基础上波动。使用RT-PCR评估已知影响细胞周期、细胞信号传导途径、碳水化合物代谢和细胞凋亡的所选miRNA的表达。与对照组相比，在-6 °C冷冻暴露24 h后，肝胰腺中的miR-1a-1、miR-34 a和miR-29 b以及足肌肉中的miR-1a-1、miR-34 a、miR-133 a、miR-125 b、miR-29 b和miR-2a的表达发生了显著变化（P < 0.05）。另外，缺氧24 h后，肝胰腺组织中miR-1a-1、miR-210和miR-29 b的表达量以及足肌组织中miR-1a-1、miR-34 a、miR-133 a、miR-29 b和miR-2a的表达量也发生了显著变化（P <0. 05）。此外，Dicer（一种负责成熟microRNA加工的酶）的蛋白表达在冷冻和缺氧期间在足肌中以及冷冻期间在肝胰腺中增加。L.中这些miRNA表达的变化。在冰冻和缺氧条件下，珊瑚礁组织可能有助于生物体的存活。
Several recent studies of vertebrate adaptation to environmental stress have suggested roles for microRNAs (miRNAs) in regulating global suppression of protein synthesis and/or restructuring protein expression patterns. The present study is the first to characterize stress-responsive alterations in the expression of miRNAs during natural freezing or anoxia exposures in an invertebrate species, the intertidal gastropod Littorina littorea. These snails are exposed to anoxia and freezing conditions as their environment constantly fluctuates on both a tidal and seasonal basis. The expression of selected miRNAs that are known to influence the cell cycle, cellular signaling pathways, carbohydrate metabolism and apoptosis was evaluated using RT-PCR. Compared to controls, significant changes in expression were observed for miR-1a-1, miR-34a and miR-29b in hepatopancreas and for miR-1a-1, miR-34a, miR-133a, miR-125b, miR-29b and miR-2a in foot muscle after freezing exposure at −6 °C for 24 h (P < 0.05). In addition, in response to anoxia stress for 24 h, significant changes in expression were also observed for miR-1a-1, miR-210 and miR-29b in hepatopancreas and for miR-1a-1, miR-34a, miR-133a, miR-29b and miR-2a in foot muscle (P < 0.05). Moreover, protein expression of Dicer, an enzyme responsible for mature microRNA processing, was increased in foot muscle during freezing and anoxia and in hepatopancreas during freezing. Alterations in expression of these miRNAs in L. littorea tissues may contribute to organismal survival under freezing and anoxia.
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影响因子：
16
作者：
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通讯作者：
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作者：
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通讯作者：
Storey, Kenneth