Regulation of myogenic stem cell behavior by vessel cells The "menage a trois" of satellite cells, periendothelial cells and endothelial cells
Regulation of myogenic stem cell behavior by vessel cells The "menage a trois" of satellite cells, periendothelial cells and endothelial cells
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血管细胞对成肌干细胞行为的调控 卫星细胞、内皮周围细胞和内皮细胞的 "三人行"
DOI：
10.4161/cc.9.5.10851
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发表时间：
2010-03-01
期刊：
影响因子：
4.3
通讯作者：
Chazaud, Benedicte
中科院分区：
文献类型：
--
作者：
Abou-Khalil, Rana;Mounier, Remi;Chazaud, Benedicte
在骨骼肌中，负责肌肉修复的卫星细胞位于毛细血管附近。尽管血管生成很长时间以来对于肌肉修复和卫星细胞存活至关重要，但探索了血管细胞与卫星/肌原性细胞之间的细胞相互作用。我们分析了肌原性细胞，内皮细胞和包括平滑肌细胞和子宫内物质成纤维细胞的细胞之间的相互关系。我们发现内皮细胞强烈刺激肌源性细胞的生长，而肌原性细胞会增加血管生成。 VEGF在这种双向相互作用中起着至关重要的作用。相反，周期细胞细胞促进了肌肉前体细胞子集的静止，以确保对成年肌肉干细胞的自我更新。我们已经表明，血管生成素1/TIE-2信号传导控制进入静止。我们提出，在肌肉再生期间，即，尽管没有稳定血管，但内皮细胞和肌源性细胞相互相互作用以促进肌发生和血管生成，而这些模型已被证明是几种模型中的过程。另一方面，一旦达到了肌肉的体内平衡，卫星细胞和友善细胞的接近度就可以使卫星细胞（带有TIE-2受体的卫星细胞）的响应能力，这些卫星细胞对生友好细胞的分泌，这些细胞在同一细胞中分泌了Angiopoietin-1。时间，通过促进内皮细胞的静止来稳定血管。
In skeletal muscle, satellite cells, that are responsible of muscle repair, are localized close to capillaries. Although angiogenesis is known for a long time to be crucial for muscle repair and satellite cell survival, cellular interplays between vessel cells and satellite/myogenic cells have been poorly explored. We analyzed the interrelationships between myogenic cells, endothelial cells, and periendothelial cells that includes smooth muscle cells and endomysial fibroblasts. We found that endothelial cells strongly stimulate myogenic cell growth and, inversely, myogenic cells increase angiogenesis. VEGF plays an essential role in this bidirectional interaction. On the contrary, periendothelial cells promote the return to quiescence of a subset of muscle precursor cells that ensures self-renewal of adult muscle stem cells. We have shown that Angiopoietin-1/Tie-2 signaling controls the entry into quiescence. We propose that during muscle regeneration, i.e., while vessels are not stabilized, endothelial cells and myogenic cells interact with each other to promote both myogenesis and angiogenesis, that have been shown to be concomitant processes in several models. On the other hand, once homeostasis of muscle is reached, the proximity of satellite cells and periendothelial cells allows the responsiveness of satellite cells, that bear Tie-2 receptor, to the secretion of Angiopoietin-1 by periendothelial cells, that, in the same time, stabilize vessels by promoting quiescence of endothelial cells.