Droplets as reaction compartments for protein nanotechnology.
Droplets as reaction compartments for protein nanotechnology.
复制标题
DOI：
10.1007/978-1-62703-354-1_16
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Hollfelder, Florian
中科院分区：
文献类型：
--
作者：
Devenish, Sean R A;Kaltenbach, Miriam;Hollfelder, Florian
在皮科至纳升的微滴中进行生物和化学反应的极端微型化正在成为一种实验范式，它使更多的实验能够以低得多的样品消耗进行，为高通量实验铺平了道路。该综述为蛋白质科学家提供了一个实验框架，用于（a）通过乳化形成多分散液滴，或者使用微流体装置形成单分散液滴;（B）为此目的构建实验装置和微流体芯片;和（c）液滴的处理和分析。
Extreme miniaturization of biological and chemical reactions in pico- to nanoliter microdroplets is emerging as an experimental paradigm that enables more experiments to be carried out with much lower sample consumption, paving the way for high-throughput experiments. This review provides the protein scientist with an experimental framework for (a) formation of polydisperse droplets by emulsification or, alternatively, of monodisperse droplets using microfluidic devices; (b) construction of experimental rigs and microfluidic chips for this purpose; and (c) handling and analysis of droplets.