Telomere measurement by quantitative PCR
Telomere measurement by quantitative PCR
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DOI：
10.1093/nar/30.10.e47
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发表时间：
2002-05-15
影响因子：
14.9
通讯作者：
Cawthon, RM
中科院分区：
文献类型：
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作者：
Cawthon, RM
长期以来，人们一直认为不可能通过设计用于与TTAGGG和CCCTAA重复序列杂交的寡核苷酸引物进行PCR扩增来测量脊椎动物DNA的端粒，因为只期望引物二聚体衍生的产物。在这里，我们提出了一个引物对，消除了这个问题，允许端粒在封闭管，荧光为基础的测定简单和快速的测量。这项检测将有助于研究端粒的生物学及其在疾病和衰老的分子病理生理学中所起的作用。
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.