Effects of host plant species on fitness costs of Bt resistance in Helicoverpa armigera (Lepidoptera: Noctuidae)
Effects of host plant species on fitness costs of Bt resistance in Helicoverpa armigera (Lepidoptera: Noctuidae)
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DOI：
10.1016/j.biocontrol.2006.11.004
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发表时间：
2007-02-01
影响因子：
4.2
通讯作者：
Akhurst, Raymond J.
中科院分区：
文献类型：
--
作者：
Bird, Lisa J.;Akhurst, Raymond J.
与抗性相关的适应性成本的幅度和主导性的增加被认为是延迟或防止昆虫种群中抗性等位基因频率增加的关键因素。我们测试了以下假设：健身成本的大小和主导性可能会受到避难所使用的农作物的影响。将棉铃虫 (Hubner) 对三种庇护植物（高粱、棉花和木豆）的性能与敏感实验室菌株、实验室选择的抗苏云金芽孢杆菌 Cry1Ac 内毒素的近等基因菌株以及这两个菌株的后代 F 进行了比较。与易感植物相比，抗性个体在每种植物上的存活率较低。与敏感品系相比，抗性品系的幼虫发育在棉花上延迟了4.7天，在高粱上延迟了1.5天，而在木豆上则没有延迟。这些结果表明，适应度成本与 Cry1Ac 抗性相关，并且对于某些性状，成本的大小取决于植物物种。一般来说，易感昆虫和以木豆为食的 F 昆虫的生活史特征相似。然而，在棉花上，与易感昆虫相比，F，昆虫的发育较慢，而在高粱上，与易感昆虫相比，F，昆虫的蛹重量和产卵量降低。这些结果表明，了解宿主避难所植物和与棉铃虫 Cry I A 抗性相关的适应性成本之间的相互作用，可能有利于最大化用于生物杀虫剂抗性管理的避难所的效益，无论是微生物植物还是转基因植物。尽管需要进一步的总体适应性数据来提供明确的立场，但数据表明，当使用苏云金芽孢杆菌或其毒素来控制棉花上的棉铃虫时，棉花可能是最有效的避难所。皇冠版权所有 (c) 2006 由 Elsevier Inc. 出版。保留所有权利。
Increases in the magnitude and dominance of fitness costs associated with resistance have been implicated as key factors in delaying or preventing increases in resistance allele frequency in insect populations. We tested the hypotheses that the magnitude and dominance of fitness costs can be affected by the crop used in the refuge. The performance of Helicoverpa armigera (Hubner) on three refuge plant species (sorghum, cotton, and pigeon pea) was compared for a susceptible laboratory strain, a near-isogenic strain selected in the laboratory against the Cry1Ac endotoxin of Bacillus thuringiensis, and the F, progeny of these two strains. Survival on each plant species was lower in resistant individuals compared with their susceptible counterparts. Compared with the susceptible strain, larval development of the resistant strain was delayed on cotton (by 4.7 days) and on sorghum (by 1.5 days), with no delay on pigeon pea. These results indicate that a fitness cost is associated with Cry1Ac resistance and that, for some traits, the magnitude of the cost depends on plant species. Generally, life history traits were similar for susceptible and F, insects that developed on pigeon pea. However, on cotton, development was slower in F, insects compared with susceptible insects, while on sorghum pupal weight and fecundity were reduced in F, insects compared with susceptible insects. These results suggest that an understanding of the interaction between host refuge plant and fitness costs associated with Cry I A resistance in H. armigera could be advantageous for maximizing the benefits of refuges used in resistance management of bioinsecticides, whether microbial or transgenic plants. Although further data on overall fitness are required to provide a definitive position, the data indicate that cotton may be the most effective refuge when B. thuringiensis or its toxins are used for controlling H. armigera on cotton. Crown copyright (c) 2006 Published by Elsevier Inc. All rights reserved.