Association of increased interferon-inducible gene expression with disease activity and lupus nephritis in patients with systemic lupus erythematosus
Association of increased interferon-inducible gene expression with disease activity and lupus nephritis in patients with systemic lupus erythematosus
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DOI：
10.1002/art.22044
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发表时间：
2006-09-01
影响因子：
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通讯作者：
Tsao, Betty P.
中科院分区：
文献类型：
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作者：
Feng, Xuebing;Wu, Hui;Tsao, Betty P.
目标。目的研究系统性红斑狼疮(SLE)患者I型干扰素诱导基因(LY6E、OAS1、OASL、MX1和ISG15)的表达水平与疾病活动性和/或临床表现的关系。从48例SLE患者、48例正常人和22例风湿病患者的外周血细胞中提取总RNA，逆转录成互补DNA。通过实时聚合酶链式反应测量基因表达水平，将其标准化为管家基因，并求和为干扰素评分。疾病活动性通过雌激素在红斑狼疮中的安全性：国家评估-系统性红斑狼疮疾病活动指数(SELENA-SLEDAI)组合来确定。SLE患者各基因表达水平均高于正常对照组(P<0.05)。
Objective. To study 5 type I interferon (IFN)-inducible genes (LY6E, OAS1, OASL, MX1, and ISG15) in patients with systemic lupus erythematosus (SLE) and to correlate expression levels with disease activity and/or clinical manifestations.Methods. Peripheral blood cells were obtained from 48 SLE patients, 48 normal controls, and 22 rheumatic disease controls, and total RNA was extracted and reverse transcribed into complementary DNA. Gene expression levels were measured by real-time polymerase chain reaction, standardized to a housekeeping gene, and summed to an IFN score. Disease activity was determined by the Safety of Estrogens in Lupus Erythematosus: National Assessment-Systemic Lupus Erythematosus Disease Activity Index (SELENA-SLEDAI) composite.Results. Each gene was highly expressed in SLE patients compared with normal controls (P