A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells
A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells
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一种基于萘酰亚胺的新型荧光探针，用于细菌和细胞中硝基还原酶 (NTR) 的成像
DOI：
10.1016/j.bmc.2019.115280
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发表时间：
2020
影响因子：
3.5
通讯作者：
Shiguo Sun
中科院分区：
文献类型：
--
作者：
Zijian Zhang;Ting Lv;Binbin Tao;Zhenfu Wen;Yongqian Xu;Hongjuan Li;Fengyu Liu;Shiguo Sun
以对硝基苄基为识别基团，1，8-萘二甲酰亚胺为荧光团，合成了一种硝基还原酶（NTR）响应性荧光探针Na-NO2，在300 μM NADH存在下，在DMSO/H2O（1：19，v/v）缓冲溶液中，NTR对Na-NO2具有明显的荧光“开启”信号。该探针的检测限低至3.4ng/mL，对大肠杆菌NTR具有很高的灵敏度。coli）、金黄色葡萄球菌（S. aureus）、正常和肿瘤细胞如HL-7702、HepG-2和MCF-7。
A nitroreductase (NTR) responsive fluorescent probe,Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized.Na-NO2showed remarkable fluorescence “turn-on” signal in the presence ofNTRunder DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence ofNADH(300 µM). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards theNTRin Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7.