Bile-induced 'pili' in Campylobacter jejuni are bacteria-independent artifacts of the culture medium
Bile-induced 'pili' in Campylobacter jejuni are bacteria-independent artifacts of the culture medium
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DOI：
10.1046/j.1365-2958.2001.02341.x
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发表时间：
2001-03-01
影响因子：
3.6
通讯作者：
Falkow, S
中科院分区：
文献类型：
--
作者：
Gaynor, EC;Ghori, N;Falkow, S
在1996年，据报道，肠道病原体空肠弯曲杆菌响应于胆汁盐如脱氧胆酸盐（DOC）产生菌毛样附件，并且这些附件的形成需要推定的肽酶PspA。已知皮利是其他病原菌的重要毒力决定因子，但以前从未在C.空肠。我们在这里报告说，这些附属物不是皮利，而是一个细菌的独立的形态学人工制品的生长培养基。此外，它们的形成不需要pspA基因。在含有临界浓度DOC的肉汤培养基中，不接种任何细菌，产生与接种C.空肠。这些纤维也发现在生长培养基中含有DOC的pspA：：Cm-R突变培养物。这些结果与蛋白质凝胶分析中缺乏候选菌毛蛋白单体以及C.空肠基因组。
In 1996, it was reported that the enteric pathogen Campylobacter jejuni produces pilus-like appendages in response to bile salts such as deoxycholate (DOC), and that the formation of these appendages requires the putative peptidase PspA. Pili were known to be important virulence determinants in other pathogenic bacteria but had never before been observed for C. jejuni. We report here that these appendages are not pili, but are instead a bacteria-independent morphological artifact of the growth medium. Furthermore, the pspA gene is not required for their formation. Broth cultures containing a threshold concentration of DOC inoculated with no bacteria produced identical abundant, fibrous, pilus-like structures as those cultures that had been inoculated with C. jejuni. These fibres were also found in growth media from DOC-containing pspA::Cm-R mutant cultures. These results are consistent with the absence of candidate pilin monomers in protein gel analyses as well as the dearth of pilin-like genes and pilus formation gene clusters in the C. jejuni genome.