Expression of a novel DnaJA1 alternative splicing in human testis and sperm
Expression of a novel DnaJA1 alternative splicing in human testis and sperm
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DOI：
10.1111/j.1365-2605.2004.00492.x
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发表时间：
2004-12-01
影响因子：
--
通讯作者：
Sha, JH
中科院分区：
文献类型：
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作者：
Hu, YQ;Zhou, ZM;Sha, JH
利用人睾丸cDNA文库的基因芯片杂交技术，将一个在成人和胚胎睾丸中表达水平差异3倍的基因命名为nDna JA1(人Dna JA1的一个新的替代亚型，也称为HDJ2/Hsdj/DJ2，人Hsp40同源物)，该基因被认为与睾丸发育和精子发生有关。多重组织聚合酶链式反应结果显示，nDNAjA1在睾丸和肺组织中高表达，在胸腺、前列腺、结肠和肝脏中低表达。对Dna JA1家族其他成员(GenBank登录号：D13388和BC008182)的结果表明，它们广泛表达。逆转录聚合酶链式反应显示，D13388和BC008182在精子中高表达，而nDnJA1在精子中弱表达。对nDNAjA1序列的蛋白质基序分析揭示了DNAJ的基序。NDnJA1和DnAJA1家族的其他成员一样，被认为是I型DNAJ。
Using cDNA microarray hybridization from a human testicular cDNA library, one gene exhibiting threefold difference at expression level between adult and embryo human testes was named nDnaJA1 (a new alternative isoform of human DnaJA1 which was also named HDJ2/Hsdj/dj2, a human HSP40 homologue), which was believed to be involved in testis development and spermatogenesis. Multiple tissue polymerase chain reaction (PCR) results showed that nDnajA1 expressed highly in testis and lung but low in thymus, prostate, colon and liver. The results of the other members of DnaJA1 family(GenBank accession numbers: D13388 and BC008182) showed that they were widely expressed. D13388 and BC008182 were highly expressed in sperm while nDnJA1 was faintly expressed by reverse transcriptase PCR. Protein motif analysis of nDnajA1 sequence revealed motifs of DnaJ. nDnJA1 was considered as type I DnaJ like the other members of DnaJA1 family.