The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development:: Effect of β-mercaptoethanol, cysteine and cystine
The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development:: Effect of β-mercaptoethanol, cysteine and cystine
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DOI：
10.1016/s0093-691x(99)00278-2
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发表时间：
2000-02-01
期刊：
影响因子：
2.8
通讯作者：
Furnus, CC
中科院分区：
文献类型：
--
作者：
de Matos, DG;Furnus, CC
在 IVM 培养基中添加半胱胺、β-巯基乙醇、半胱氨酸和胱氨酸诱导牛卵母细胞谷胱甘肽 (GSH) 合成，但仅测试了半胱胺对这些卵母细胞发育能力的影响。在绵羊卵母细胞 IVM 过程中，半胱胺而非 β-巯基乙醇促进了胚胎发育。然而，尚不清楚使用硫醇化合物进行 IVM 后获得的高细胞内卵母细胞 GSH 水平可以维持多久。因此，本研究旨在评估在成熟培养基中添加100μMβ-巯基乙醇、0.6mM半胱氨酸和0.6mM胱氨酸对1）IVM后细胞内GSH水平的影响，2）IVF后，3）6至8细胞胚胎中的细胞内GSH水平以及4）对胚胎发育的影响。在 IVM 后的卵母细胞和 IVF 后的推定受精卵中，治疗组的细胞内 GSH 水平显着升高 (P0.05)。与在对照培养基中成熟的卵母细胞相比，处理过的卵母细胞的卵裂率以及发育至桑葚胚和囊胚阶段的胚胎百分比的差异显着更高(P<0.05)。我们从结果中得出结论，硫醇化合物诱导牛 IVM 中 GSH 合成后的高细胞内 GSH 水平在 IVF 期间仍然存在，并且在 IVC 开始时仍然存在，从而提高了发育速度。此外，结果表明该代谢途径是细胞质成熟过程的重要组成部分，影响体外胚胎生产的后续步骤。 (C) 2000 年，爱思唯尔科学公司。
Supplementation of IVM medium with cysteamine, beta-mercaptoethanol, cysteine and cystine induced bovine oocyte glutathione (GSH) synthesis, but only the effect of cysteamine on the developmental competence of these oocytes was tested. During IVM of sheep oocytes, cysteamine but not beta-mercaptoethanol increased embryo development. However, it is not known how long the high intracellular oocyte GSH levels obtained after IVM with thiol compounds, can be maintained. Thus, the present study was carried out to evaluate the effects of supplementing maturation medium with 100 mu M beta-mercaptoethanol, 0.6 mM cysteine and 0.6 mM cystine on 1) intracellular GSH level after IVM, 2) after IVF, 3) in 6 to 8-cell embryos and 4) on embryo development. In oocytes after IVM and in presumptive zygotes after IVF, intracellular GSH levels were significantly higher in the treated groups (P0.05). Differences in cleavage rates and the percentage of embryos that developed to morula and blastocyst stages were significantly higher (P< 0.05) for treated oocytes than for those matured in the control medium. We conclude from the results that the high intracellular GSH levels after induction of GSH synthesis in bovine IVM by thiol compounds remain during IVF and are still present at the beginning of IVC, improving developmental rates. Moreover, the results indicate that this metabolic pathway is an important component of the cytoplasmic maturation process that affects the subsequent steps of in vitro embryo production. (C) 2000 by Elsevier Science Inc.