Rhizobium alkalisoli sp nov., isolated from Caragana intermedia growing in saline-alkaline soils in the north of China
Rhizobium alkalisoli sp nov., isolated from Caragana intermedia growing in saline-alkaline soils in the north of China
复制标题
DOI：
10.1099/ijs.0.007237-0
复制
发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Chen, Wen Xin
中科院分区：
文献类型：
--
作者：
Lu, Yang Li;Chen, Wen Feng;Chen, Wen Xin
从我国北方盐碱地中间锦鸡儿根瘤中分离到的3株根瘤菌（CCBAU 01393（T）、CCBAU 01389和CCBAU 03239）的16 S rRNA基因序列与美国大豆根瘤菌（Rhizobium huautobium SO2（T））和美国大豆根瘤菌（Rhizobium galegae USDA 4128（T））的同源性分别为99.7%和99.5%。管家基因atpD，recA和glnII的系统发育证实了它们的独特位置，不同于公认的根瘤菌物种。全细胞可溶性蛋白的SDS-PAGE和一系列的表型和生理测试使我们能够区分新的组从所有密切相关的公认的根瘤菌，物种。菌株CCBAU 01393（T）与R. huautumSO_2（T）和R. galegae USDA 4128（T）的遗传变异系数分别为34.9和20.5。因此，我们认为菌株CCBAU 01393（T）、CCBAU 01389和CCBAU 03239代表一个新种，Rhizobium alkalisoli sp. nov.，以菌株CCBAU 01393（T）（=LMG 24763（T）=HAMBI 3051（T））作为模式菌株。该菌株在小叶锦鸡儿、菜豆和绿豆上均能形成有效根瘤。
Three rhizobial strains (CCBAU 01393(T), CCBAU 01389 and CCBAU 03239) isolated from nodules of Caragana intermedia grown in saline-alkaline soils in the north of China had identical 16S rRNA genes that showed 99.7 and 99.5% sequence similarities with those of Rhizobium huautlense SO2(T) and Rhizobium galegae USDA 4128(T), respectively. Phylogenies of the housekeeping genes atpD, recA and glnII confirmed their distinct position, differing from recognized Rhizobium species. SDS-PAGE of whole-cell soluble proteins and a series of phenotypic and physiological tests allowed us to differentiate the novel group from all closely related recognized Rhizobium, species. The levels of DNA-DNA relatedness between strain CCBAU 01393(T) and R. huautlense SO2(T) and R. galegae USDA 4128(T) were 34.9 and 20.5 respectively. Therefore, we propose that strains CCBAU 01393(T), CCBAU 01389 and CCBAU 03239 represent a novel species, Rhizobium alkalisoli sp. nov., with strain CCBAU 01393(T) (=LMG 24763(T) =HAMBI 3051(T)) as the type strain. This strain could form effective nodules on Caragana microphylla, Phaseolus vulgaris and Vigna radiata.