Olig genes are expressed in a heterogeneous population of precursor cells in the developing spinal cord
Olig genes are expressed in a heterogeneous population of precursor cells in the developing spinal cord
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作者：
Liu, Y;Rao, MS
最近来自多个实验室的结果已经确定了oliggenes在调节胶质细胞分化中起重要作用。本研究表明，早期发育阶段（E16.5之前）的Olig2表达确定了发育中的脊髓中的一个结构域，其中包含包括干细胞和胶质祖细胞在内的异质祖细胞群。我们发现Nkx2.2和Olig2，它们最初存在于非重叠结构域，在后期阶段共表达，可能是由于Olig2表达的第二波。我们发现Olig1和Olig2一样存在于共表达星形胶质细胞和放射状胶质标记物的细胞中，并且Olig1/2的双敲除导致少突胶质细胞的缺失，同时保留了NG2的表达。这些结果加上先前发表的数据表明，Olig1/2和Nkx2.2虽然在调节早期祖细胞分化中明显重要，但并不能明确证明少突胶质细胞-神经元前体的存在，也不能否定现有的逆转录病毒谱系和克隆分析数据，这些数据表明存在其他类型的前体，如少突胶质细胞-星形胶质细胞前体或神经元前体。2003年Wiley-Liss出版社出版
Recent results from multiple laboratories have identified Olig genes as important in regulating glial differentiation. Here we show that Olig2 expression at early stages of development (prior to E16.5) identifies a domain in the developing spinal cord, which contains a heterogeneous population of progenitors that includes stem cells and glial progenitors. We show that Nkx2.2 and Olig2, which are present initially in nonoverlapping domains, are coexpressed at later stages, likely due to a second wave of Olig expression. We find that Olig1, like Olig2, is present in cells that coexpress astrocytic and radial glial markers and that Olig1/2 double knockouts lead to a loss of oligodendrocytes with preservation of NG2 expression. These results coupled with previously published data indicate that Olig1/2 and Nkx2.2, while clearly important in regulating early progenitor cell differentiation, do not unambiguously demonstrate the existence of an oligodendrocyte-neuron precursor or negate the existing retroviral lineage and clonal analysis data that suggest the existence of other types of precursors such as oligodendrocyte-astrocyte precursors or neuronal precursors. Published 2003 Wiley-Liss, Inc.dagger