INTRACELLULAR-DISTRIBUTION OF POLIOVIRUS PROTEINS AND THE INDUCTION OF VIRUS-SPECIFIC CYTOPLASMIC STRUCTURES
INTRACELLULAR-DISTRIBUTION OF POLIOVIRUS PROTEINS AND THE INDUCTION OF VIRUS-SPECIFIC CYTOPLASMIC STRUCTURES
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DOI：
10.1016/0042-6822(83)90531-7
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发表时间：
1983-01-01
期刊：
影响因子：
3.7
通讯作者：
BOSSART, W
中科院分区：
文献类型：
--
作者：
BIENZ, K;EGGER, D;BOSSART, W
在易感细胞中，肠道病毒诱导出一个囊泡化区域（病毒诱导的囊泡），它既是病毒RNA合成的位点，也是在形态学上被称为细胞病变效应的位点。已表明脊髓灰质炎病毒（I型，Mahonney株）的蛋白质迁移到被感染的人喉癌HEp - 2细胞的病毒诱导囊泡区域。发现有五种蛋白质（P2 - 5b、P3 - 4b、P3 - 6a、P3 - 7c、P3 - 9）与囊泡本身相关联，要么作为内在膜蛋白（P3 - 9），要么以囊泡内的可溶形式（P3 - 4b、P3 - 7c以及部分P3 - 6a）存在，要么结合到一种抗脱氧胆酸钠的结构上（P2 - 5b和少量P3 - 6a）。用氯化锌部分抑制病毒多聚蛋白的裂解，以改变细胞内的病毒蛋白模式。所获得的数据表明，P2 - 5b是负责形成病毒诱导囊泡的蛋白质。
In a susceptible cell, enteroviruses induce a vesiculated region (the virus-induced vesicles) which is both the site of viral RNA synthesis as well as the site referred to morphologically, as the cytopathic effect. Proteins of poliovirus (type I, Mahoney) were shown to migrate into the region of the virus-induced vesicles of infected human laryngeal carcinoma HEp-2 cells. Five proteins (P2-5b, P3-4b, P3-6a, P3-7c, P3-9) were found to be associated with the vesicles themselves, either as intrinsic membrane protein (P3-9) or in a soluble form within the vesicles (P3-4b, P3-7c, and, partially, P3-6a) or bound to a sodium deoxycholate-resistant structure (P2-5b and a small amount of P3-6a). Partial inhibition of the cleavage of the viral polyprotein with ZnCl2 was used to alter the viral protein pattern within the cells. The data obtained indicate that P2-5b is the protein responsible for the formation of the virus-induced vesicles.