The development of quick, robust, quantitative phenotypic assays for describing the host-nonhost landscape to stripe rust.
The development of quick, robust, quantitative phenotypic assays for describing the host-nonhost landscape to stripe rust.
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DOI：
10.3389/fpls.2015.00876
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发表时间：
2015
影响因子：
5.6
通讯作者：
Moscou MJ
中科院分区：
文献类型：
--
作者：
Dawson AM;Bettgenhaeuser J;Gardiner M;Green P;Hernández-Pinzón I;Hubbard A;Moscou MJ
非宿主抗性通常被概念化为与宿主抗性的质的分离。这两种状态的分类通常很容易，因为它们不能完全描述从宿主到非宿主的转变中存在的状态范围。当研究不能被分类为宿主或非宿主的病理系统时，由于它们相对于这两个极端的中间状态，这就提出了一个问题。在这项研究中，我们调查的效力Poaceae条锈病（Puccinia striiformis韦斯滕特。用于描述宿主-非宿主景观的交互。首先，以大麦（Hordeum vulgare L.）和二穗短柄草Brachypodium distachyon（L.）P. Beauv。我们观察到，黄化和叶片布朗宁的宏观症状与菌丝定殖有关。sp. triangulae的相似性。这促使我们采用一种将真菌结构可视化的方案进行表型测定，以估计叶片定殖的百分比。在中间宿主和中间非宿主系统中使用该检测发现，感染频率随着与宿主物种的进化分化而降低。同样，我们观察到病原体完成其生命周期的能力比其在叶组织上定殖的能力下降得更快，与宿主系统相比，在中间非宿主系统中没有观察到脓包的发生，并且显着减少了中间宿主系统中脓包的形成，大麦-P. striiformis f.大麦锈病菌通过利用条锈病致病系统结合宏观和微观表型测定，我们现在希望解剖中间宿主和中间非宿主抗性的遗传结构，在大麦和B中使用结构化群体。二穗子
Nonhost resistance is often conceptualized as a qualitative separation from host resistance. Classification into these two states is generally facile, as they fail to fully describe the range of states that exist in the transition from host to nonhost. This poses a problem when studying pathosystems that cannot be classified as either host or nonhost due to their intermediate status relative to these two extremes. In this study, we investigate the efficacy of the Poaceae-stripe rust (Puccinia striiformis Westend.) interaction for describing the host–nonhost landscape. First, using barley (Hordeum vulgare L.) and Brachypodium distachyon (L.) P. Beauv. We observed that macroscopic symptoms of chlorosis and leaf browning were associated with hyphal colonization by P. striiformis f. sp. tritici, respectively. This prompted us to adapt a protocol for visualizing fungal structures into a phenotypic assay that estimates the percent of leaf colonized. Use of this assay in intermediate host and intermediate nonhost systems found the frequency of infection decreases with evolutionary divergence from the host species. Similarly, we observed that the pathogen’s ability to complete its life cycle decreased faster than its ability to colonize leaf tissue, with no incidence of pustules observed in the intermediate nonhost system and significantly reduced pustule formation in the intermediate host system as compared to the host system, barley-P. striiformis f. sp. hordei. By leveraging the stripe rust pathosystem in conjunction with macroscopic and microscopic phenotypic assays, we now hope to dissect the genetic architecture of intermediate host and intermediate nonhost resistance using structured populations in barley and B. distachyon.