In situ azide formation and "click" reaction of nile red with DNA as an alternative postsynthetic route
In situ azide formation and "click" reaction of nile red with DNA as an alternative postsynthetic route
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DOI：
10.1039/b924471a
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发表时间：
2010-01-01
影响因子：
4.9
通讯作者：
Wagenknecht, Hans-Achim
中科院分区：
文献类型：
--
作者：
Beyer, Christoph;Wagenknecht, Hans-Achim
在序列中含有5-碘-2 ′-脱氧尿苷的寡核苷酸可以通过原位形成中间体叠氮化物，用乙炔基修饰的尼罗红作为荧光标记进行合成后“点击”型修饰。
Oligonucleotides that contain a 5-iodo-2'-deoxyuridine in the sequence can be applied for a postsynthetic "click"-type modification with ethynyl-modified nile red as a fluorescent label by in situ formation of the intermediate azide.