Genetic control by a metabolite binding mRNA
Genetic control by a metabolite binding mRNA
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DOI：
10.1016/s1074-5521(02)00224-7
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发表时间：
2002-09-01
影响因子：
--
通讯作者：
Breaker, RR
中科院分区：
文献类型：
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作者：
Nahvi, A;Sudarsan, N;Breaker, RR
信使RNA通常被认为是遗传信息的被动载体，在翻译过程中受到蛋白质或小RNA调节因子和核糖体的作用。我们报告说，大肠杆菌btuB基因的5‘非翻译序列在代谢监测和遗传控制中具有更积极的作用。该信使核糖核酸通过选择性结合辅酶B-12而不需要蛋白质，起到了代谢物感应遗传开关的作用。这种结合事件建立了一种独特的RNA结构，可能负责抑制核糖体结合，从而减少钴胺运输蛋白BtuB的合成。这一发现以及相关的观察结果支持了这样一种假设，即通过RNA-代谢物相互作用进行代谢监测是一种广泛存在的遗传控制机制。
Messenger RNAs are typically thought of as passive carriers of genetic information that are acted upon by protein- or small RNA-regulatory factors and by ribosomes during the process of translation. We report that the 5'-untranslated sequence of the Escherichia coli btuB mRNA assumes a more proactive role in metabolic monitoring and genetic control. The mRNA serves as a metabolite-sensing genetic switch by selectively binding coenzyme B-12 without the need for proteins. This binding event establishes a distinct RNA structure that is likely to be responsible for inhibition of ribosome binding and consequent reduction in synthesis of the cobalamin transport protein BtuB. This finding, along with related observations, supports the hypothesis that metabolic monitoring through RNA-metabolite interactions is a widespread mechanism of genetic control.