A survey of liquid chromatographic-mass spectrometric analysis of mercapturic acid biomarkers in occupational and environmental exposure monitoring.
A survey of liquid chromatographic-mass spectrometric analysis of mercapturic acid biomarkers in occupational and environmental exposure monitoring.
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DOI：
10.1016/j.jchromb.2014.02.057
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发表时间：
2014-08-01
期刊：
影响因子：
--
通讯作者：
B'Hymer C
中科院分区：
文献类型：
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作者：
Mathias PI;B'Hymer C
高效液相色谱/质谱仪(HPLC/MS)是一种灵敏、特异的靶向定量分析方法，可用于生物基质中的小分子物质分析。本文简要介绍了最近选定的高效液相色谱/质谱法测定尿中的硫代尿酸(硫代尿酸)的方法，这些方法在职业和环境研究中可作为生物标志物用于表征人体接触亲电工业化学品的特征。亲电化合物因其反应性而被用于化学和工业过程。它们存在于工业废气中，是化石燃料的燃烧产物，也是烟草烟雾中的成分。它们在工业和一般环境中的存在是对人类和环境健康的关切。尿硫藻酸盐是活性化学物质代谢解毒的产物，为研究人体接触亲电性毒物提供了一种非侵入性工具。总结了最新的硫代碳酸酯定量方法，并给出了具体的案例。介绍了硫代硫酸酯的生物形成，并讨论了它们作为亲电性化合物代谢过程的生物标志物的应用。此外，还讨论了在一次测定中同时测定多个母体化合物的硫代碳酸酯的使用，以及这一研究领域的未来趋势和局限性。
High-performance liquid chromatography/mass spectrometry (HPLC/MS) is sensitive and specific for targeted quantitative analysis and is readily utilized for small molecules from biological matricies. This brief review describes recent selected HPLC/MS methods for the determination of urinary mercapturic acids (mercapturates) which are useful as biomarkers in characterizing human exposure to electrophilic industrial chemicals in occupational and environmental studies. Electrophilic compounds owing to their reactivity are used in chemical and industrial processes. They are present in industrial emissions, are combustion products of fossil fuels, and are components in tobacco smoke. Their presence in both the industrial and general environment are of concern for human and environmental health. Urinary mercapturates which are the products of metabolic detoxification of reactive chemicals provide a non-invasive tool to investigate human exposure to electrophilic toxicants. Selected recent mercapturate quantification methods are summarized and specific cases are presented. The biological formation of mercapturates is introduced and their use as biomarkers of metabolic processing of electrophilic compounds is discussed. Also, the use of liquid chromatography/tandem mass spectrometry in simultaneous determinations of the mercapturates of multiple parent compounds in a single determination is considered, as well as future trends and limitations in this area of research.