The dpsA gene of Streptomyces coelicolor: induction of expression from a single promoter in response to environmental stress or during development.
The dpsA gene of Streptomyces coelicolor: induction of expression from a single promoter in response to environmental stress or during development.
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DOI：
10.1371/journal.pone.0025593
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Del Sol R
中科院分区：
文献类型：
--
作者：
Facey PD;Sevcikova B;Novakova R;Hitchings MD;Crack JC;Kormanec J;Dyson PJ;Del Sol R
DpsA蛋白在天蓝色链霉菌中起着双重作用，既作为应激反应的一部分，又有助于孢子形成过程中的类核凝聚。启动子定位实验表明，dpsA是从一个单一的，sigB样依赖性启动子转录。表达研究暗示SigH和SigB作为负责dpsA表达的sigma因子，而其他SigB样因子的贡献是间接的，通过控制sigH表达。该启动子响应于渗透胁迫而被大量诱导，部分原因是其对DNA超螺旋变化的敏感性。此外，我们确定了WhiB是dpsA表达所必需的，特别是在发育过程中。凝胶阻滞实验揭示了apoWhiB和dpsA启动子区域之间的直接相互作用，为S.天蓝色。
The DpsA protein plays a dual role in Streptomyces coelicolor, both as part of the stress response and contributing to nucleoid condensation during sporulation. Promoter mapping experiments indicated that dpsA is transcribed from a single, sigB-like dependent promoter. Expression studies implicate SigH and SigB as the sigma factors responsible for dpsA expression while the contribution of other SigB-like factors is indirect by means of controlling sigH expression. The promoter is massively induced in response to osmotic stress, in part due to its sensitivity to changes in DNA supercoiling. In addition, we determined that WhiB is required for dpsA expression, particularly during development. Gel retardation experiments revealed direct interaction between apoWhiB and the dpsA promoter region, providing the first evidence for a direct WhiB target in S. coelicolor.
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影响因子：
3.2
作者：
Antelmann, H;Engelmann, S;Hecker, M
通讯作者：
Hecker, M
影响因子：
2.1
作者：
Flett, F;Mersinias, V;Smith, CP
通讯作者：
Smith, CP
影响因子：
2.8
作者：
Mazurakova, Vladislava;Sevcikova, Beatrica;Kormanec, Jan
通讯作者：
Kormanec, Jan
影响因子：
3.6
作者：
Kelemen, GH;Viollier, PH;Thompson, CJ
通讯作者：
Thompson, CJ
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF