Expression of Neuropeptide FF Defines a Population of Excitatory Interneurons in the Superficial Dorsal Horn of the Mouse Spinal Cord that Respond to Noxious and Pruritic Stimuli
Expression of Neuropeptide FF Defines a Population of Excitatory Interneurons in the Superficial Dorsal Horn of the Mouse Spinal Cord that Respond to Noxious and Pruritic Stimuli
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DOI：
10.1016/j.neuroscience.2019.08.013
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发表时间：
2019-09-15
期刊：
影响因子：
3.3
通讯作者：
Todd, Andrew J.
中科院分区：
文献类型：
--
作者：
Gutierrez-Mecinas, Maria;Bell, Andrew;Todd, Andrew J.
脊髓背角浅层的绝大多数神经元是兴奋性中间神经元，它们是正常感知疼痛和瘙痒所必需的。我们以前已经确定了5个基本上不重叠的人口在这些细胞中，基于四种不同的神经肽（胆囊收缩素，神经降压素，神经激肽B和P物质）的表达和绿色荧光蛋白的启动子驱动的胃泌素释放肽（GRP）在转基因小鼠系。另一种肽（神经肽FF，NPFF）已在兴奋性神经元中鉴定，在这里，我们使用了针对NPFF前体（proNPFF）的抗体和识别Npff mRNA的探针来鉴定和鉴定这些细胞。我们发现，它们都是兴奋性中间神经元，并从上述五个群体中分离出来，约占I-II层兴奋性神经元的6%。通过检测细胞外信号调节激酶的磷酸化，我们发现NPFF细胞可以对不同类型的伤害性和刺激性刺激作出反应。已显示切除表达生长抑素的背角神经元导致机械疼痛敏感性的显著降低，而从这些神经元释放的生长抑素被认为有助于瘙痒。由于绝大多数NPFF细胞共表达生长抑素，这些细胞可能在疼痛和瘙痒的感知中发挥作用。(C)2019作者（S）由Elsevier Ltd代表IBRO出版。
The great majority of neurons in the superficial dorsal horn of the spinal cord are excitatory interneurons, and these are required for the normal perception of pain and itch. We have previously identified 5 largely non-overlapping populations among these cells, based on the expression of four different neuropeptides (cholecystokinin, neurotensin, neurokinin B and substance P) and of green fluorescent protein driven by the promoter for gastrin-releasing peptide (GRP) in a transgenic mouse line. Another peptide (neuropeptide FF, NPFF) has been identified among the excitatory neurons, and here we have used an antibody against the NPFF precursor (proNPFF) and a probe that recognises Npff mRNA to identify and characterise these cells. We show that they are all excitatory interneurons, and are separate from the five populations listed above, accounting for similar to 6% of the excitatory neurons in laminae I-II. By examining phosphorylation of extracellular signal-regulated kinases, we show that the NPFF cells can respond to different types of noxious and pruritic stimulus. Ablation of somatostatin-expressing dorsal horn neurons has been shown to result in a dramatic reduction in mechanical pain sensitivity, while somatostatin released from these neurons is thought to contribute to itch. Since the great majority of the NPFF cells co-expressed somatostatin, these cells may play a role in the perception of pain and itch. (C) 2019 The Author(s). Published by Elsevier Ltd on behalf of IBRO.