A precision flow-controlled Rb-82 generator for bolus or constant-infusion studies of the heart and brain.
A precision flow-controlled Rb-82 generator for bolus or constant-infusion studies of the heart and brain.
复制标题
精密流量控制 Rb-82 发生器，用于心脏和大脑的推注或持续输注研究。
DOI：
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发表时间：
1981
影响因子：
9.3
通讯作者：
T. Budinger
中科院分区：
文献类型：
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作者：
Y. Yano;J. Cahoon;T. Budinger
一个精密的流动控制铷-82发生器已经建成提供76秒的铷-82无论是快或慢的流速为大剂量或恒定输注的研究。步进电机驱动器连接到微处理器，用于脉冲控制流速，从大容量（150毫升）机械泵送注射器通过氧化铝柱输送生理盐水洗脱液，该柱保留25天Sr-82母体。生成系统在20-25 ml 2% NaCl溶液中提供70-90%的最大Rb-82活性。Sr-82/Sr-85的突破是10(-7)- 10（-6）。产量和突破都是柱长和流量的函数。在近2年的时间里，在心肌血液灌注和血脑屏障通透性变化的研究中，对发电机的6个单独的Sr-82负荷进行了评估。Rb-82洗脱液在多次洗脱和每次发电机加载3-4个月的长期使用中保持无菌性和无原性。
A precision flow-controlled rubidium-82 generator has been constructed to deliver 76-sec Rb-82 at either fast or slow flow rates for bolus or constant-infusion studies. A stepping motor drive is interfaced to a microprocessor for pulsed control of flow rate to deliver the saline eluant solution from a large-volume (150 ml) machined pumping syringe through an alumina column that retains the 25-day Sr-82 parent. The generator system delivers 70-90% of the maximum Rb-82 activity in a 20-25 ml bolus elution of 2% NaCl. The Sr-82/Sr-85 breakthrough is 10(-7) - 10(-6). both yield and breakthrough are functions of column length and flow rate. Six separate Sr-82 loadings of the generator were evaluated over a period of nearly 2 yr in studies of myocardial blood perfusion and permeability changes in the blood-brain barrier. Sterility and apyrogenicity of the Rb-82 eluate were maintained during multiple elutions and long-term use of 3-4 mo for each generator loading.