EVIDENCE THAT THE GLUCOSE TRANSPORTER SERVES AS A WATER CHANNEL IN J774 MACROPHAGES
EVIDENCE THAT THE GLUCOSE TRANSPORTER SERVES AS A WATER CHANNEL IN J774 MACROPHAGES
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DOI：
10.1073/pnas.86.21.8397
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发表时间：
1989-11-01
影响因子：
11.1
通讯作者：
LOIKE, J
中科院分区：
文献类型：
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作者：
FISCHBARG, J;KUANG, K;LOIKE, J
水的跨膜转运是细胞的一个普遍性质，但这种转运的途径尚不清楚。在这项研究中，通过记录细胞散射的光强度来监测J774鼠巨噬细胞样细胞系的细胞体积变化。我们研究了几种葡萄糖转运抑制剂对细胞膜渗透性水渗透性的影响，从细胞体积变化率计算。细胞松弛素B（2.5 μ g/ml）、根皮素（20 μ M）和番茄碱（3 μ M）可逆地阻断葡萄糖摄取进入这些细胞。所有三种抑制剂可逆地降低了J774细胞的渗透水渗透压，从89.6 ± 0.01 μ g/ml。3.2至27.2 .+-。1.4μ m/sec。我们的结论是，渗透水流经这些细胞的质膜的主要组成部分是占水穿越他们的葡萄糖转运蛋白。
Water transport across plasma membrane is a universal property of cells, but the route of such transport is unclear. In this study, volume changes of cells of the J774 murine macrophage-like cell line were monitored by recording the intensity of light scattered by the cells. We investigated the effects of several inhibitors of glucose transport on cell membrane osmotic water permeability as calculated from the rates of cell volume change. Cytochalasin B (2.5 .mu.g/ml), phloretin (20 .mu.M), and tomatine (3 .mu.M) reversibly blocked glucose uptake into these cells. All three inhibitors reversibly decreased the osmotic water pemeability of J774 cells from 89.6 .+-. 3.2 to 27.2 .+-. 1.4 .mu.m/sec. We conclude that a major component of the osmotic water flow across the plasma membranes of these cells is accounted for by water traversing their glucose transporters.