Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction.
Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction.
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DOI：
10.1016/j.jbiotec.2009.10.011
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发表时间：
2010
影响因子：
4.1
通讯作者：
Tsutomu Tanaka;Shinsuke Masunari;Jun Ishii;K. Wakamura;Maiko Segawa;H. Fukuda;A. Kondo
中科院分区：
文献类型：
--
作者：
Tsutomu Tanaka;Shinsuke Masunari;Jun Ishii;K. Wakamura;Maiko Segawa;H. Fukuda;A. Kondo
在这里，我们扩展酵母细胞表面展示系统，展示非天然的，功能性分子。对大肠杆菌生物素连接酶的生物素受体短肽（BAP）序列进行了分析。coli（BirA）中，在锚蛋白Flo 428的N-末端进行遗传导入。通过将BAP融合蛋白Flo 428与BirA共表达，可将生物素化的BAP展示在酵母细胞表面。随后添加链霉亲和素-FITC导致链霉亲和素-FITC的展示，并且使用链霉亲和素作为接头成功展示生物素-FITC。我们的策略为在酵母细胞表面展示功能分子提供了一个强有力的工具。
Here we expand the yeast cell surface display system to display non-natural, functional molecules. The short biotin acceptor peptide (BAP) sequence of biotin ligase from E. coli (BirA) was genetically introduced to the N-terminus of the anchor protein, Flo428. Through co-expression of BAP-fused Flo428 with BirA, biotinylated BAP could be displayed on the yeast cell surface. Subsequent addition of streptavidin–FITC resulted in the display of streptavidin–FITC, and, the display of biotin–FITC was successful using streptavidin as a linker. Our strategy provides a powerful tool for displaying functional molecules on yeast cell surfaces.