Staphylococcus aureus growth and enterotoxin A production in an anaerobic environment
Staphylococcus aureus growth and enterotoxin A production in an anaerobic environment
复制标题
DOI：
10.4315/0362-028x-65.1.199
复制
发表时间：
2002-01-01
影响因子：
2
通讯作者：
Rasooly, A
中科院分区：
文献类型：
--
作者：
Belay, N;Rasooly, A
研究了严格的厌氧条件对金黄色葡萄球菌生长和金黄色葡萄球菌肠毒素A(SEA)产生的影响。金黄色葡萄球菌是一种兼性厌氧细菌，厌氧生长慢于好氧生长。在37℃脑心输液上培养，中对数生长期厌氧产生期为80min，好氧对照组为35min。与之前的研究表明，葡萄球菌在需氧培养中的细胞密度是厌氧培养的9至17倍相反，与食物中毒有关的葡萄球菌菌株的数据显示，在需氧培养中的细胞密度仅为需氧培养的2至3倍。免疫印迹法检测SEA的产生，结果显示SEA的产生依赖于生长。在厌氧生长较慢的情况下，产生的毒素相对较少，但在两种情况下，培养120分钟后都检测到SEA。还研究了温度和曝气对金黄色葡萄球菌的联合影响，分析了14~37℃温度范围内好氧和厌氧培养物的生长和产毒情况。在两种环境中的低温下仍能观察到生长。根据这些研究建立了金黄色葡萄球菌有氧或厌氧生长与培养温度的线性模型。在17~35.5℃的温度范围内对该模型进行了检验，结果表明该模型能够准确地预测该温度范围内金黄色葡萄球菌的生长速率。这些数据表明，厌氧条件并不是阻止金黄色葡萄球菌生长的有效屏障。
The effects of strict anaerobic conditions on the growth of Staphylococcus aureus and the production of staphylococcal enterotoxin A (SEA) were studied. The growth of S. aureus, a facultative anaerobic bacterium, is slower anaerobically than aerobically. When grown on brain heart infusion broth at 37degreesC, the anaerobic generation time at mid-log phase was 80 min, compared with 35 min for the aerobic control. In contrast to previous studies demonstrating that staphylococcal cell density was 9- to 17-fold greater in aerobic than in anaerobic cultures, data for a staphylococcal strain implicated in food poisoning showed that the cell density was only two to three times as great in aerobic cultures. Production of SEA was monitored by Western immunoblotting and shown to be growth dependent. With slower anaerobic growth, relatively less toxin was produced than under aerobic conditions, but in both cases SEA was detected after 120 min of incubation. The combined effects of temperature and aeration on S. aureus were also studied, Growth and toxin production of aerobic and anaerobic cultures at temperatures ranging from 14 to 37degreesC were analyzed. Growth was still observed at low temperatures in both environments. A linear model for S. aureus aerobic or anaerobic growth as a function of incubation temperature was developed from these studies. The model was tested from 17 to 35.5degreesC, and the results suggest that the model can accurately predict the S. aureus growth rate in this temperature range. The data suggest that anaerobic conditions are not an effective barrier against S. aureas growth.