GONADOTROPIN RECEPTORS OF THE OVINE OVARIAN FOLLICLE DURING FOLLICULAR-GROWTH AND ATRESIA
GONADOTROPIN RECEPTORS OF THE OVINE OVARIAN FOLLICLE DURING FOLLICULAR-GROWTH AND ATRESIA
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DOI：
10.1095/biolreprod21.1.75
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发表时间：
1979-01-01
影响因子：
3.6
通讯作者：
TROUNSON, AO
中科院分区：
文献类型：
--
作者：
CARSON, RS;FINDLAY, JK;TROUNSON, AO
将[125I]标记的人卵泡刺激素（[125I]-FSH）和绒毛膜促性腺激素（[125I]-hCG）与完整绵羊卵泡的结合在体外作为卵泡直径和形态闭锁阶段的函数进行了研究。对闭锁分类进行了组织学确认，在所研究的那些卵泡中闭锁的发生率是可变的，并且与卵泡直径直接相关（P<0.01）。与随着卵泡直径增加或闭锁阶段变化相关的颗粒细胞结合变化相比，[125I]-hFSH和[125I]-hCG与卵泡膜的结合相对恒定。当不考虑闭锁阶段进行研究时，随着卵泡直径增加，[125I]-hFSH与颗粒细胞的结合减少，而[125I]-hCG的结合增加。这些变化被认为反映了每个大小组内闭锁相对发生率的变化，而非结合本身的减少。随后对[125I]标记的促性腺激素与颗粒细胞结合作为卵泡直径和闭锁阶段两者的函数同时进行分析表明，[125I]-hFSH结合的程度仅由闭锁阶段而非卵泡直径决定（P<0.05）。相反，虽然[125I]-hCG结合因闭锁增加而减少，但结合的总体程度由卵泡直径决定（P<0.01）。卵巢卵泡群中卵泡闭锁的宏观评估与卵泡直径直接相关。此外，颗粒细胞在体外结合[125I]标记的促性腺激素的能力随着卵泡直径和闭锁阶段的函数而变化。
The binding of [125I]-labeled human follicle stimulating hormone ([125I]-FSH) and chorionic gonadotropin ([125I]-hCG) to intact ovine follicles was studied in vitro as a function of follicular diameter and stage of morphological atresia. Histological confirmation of the atretic classification was established and the incidence of atresia in those follicles studies was variable and directly related to follicular diameter (P < 0.01). The binding of [125I]-hFSH and [125I]-hCG to theca was relatively constant when compared to the change in granulosa binding that was associated with increased follicular diameter or stage of atresia. When studied without regard to the stage of atresia, the binding of [125I]-hFSH to granulosa cells decreased and that of [125I]-hCG increased with increased follicular diameter. These changes were thought to reflect changes in the relative incidence of atresia within each size group rather than decreased binding per se. Subsequent analysis of [125I]-labeled gonadotropin binding to granulosa cells as a function of both follicular diameter and stage of atresia simultaneously indicated that the extent of [125I]-hFSH binding was determined solely by stage of atresia rather than follicular diameter (P < 0.05). Conversely, while [125I]-hCG binding was decreased by increased atresia, the overall extent of binding was determined by follicular diameter (P < 0.01). Macroscopic assessment of follicular atresia in ovarian follicular populations is directly related to follicular diameter. In addition, the ability of granulosa cells to bind [125I]-labeled gonadotropins in vitro varies as a function of follicular diameter and stage of atresia.