Confirmation of potential herbicidal agents in hulls of rice, Oryza sativa
Confirmation of potential herbicidal agents in hulls of rice, Oryza sativa
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DOI：
10.1007/s10886-005-5290-5
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发表时间：
2005-06-01
影响因子：
2.3
通讯作者：
Ahmad, A
中科院分区：
文献类型：
--
作者：
Chung, IM;Hahn, SJ;Ahmad, A
稻壳的乙酸乙酯提取物产生七种化合物：十三烷、1-三十四烷醇、β-谷甾醇、豆蔻内酯A、豆蔻内酯B、麦黄酮（类黄酮）和β-谷甾醇-3-O-β-D-葡糖苷。这些化合物的结构用500 MHz核磁共振（NMR），使用一维和二维光谱方法，辅助电子电离质谱（EI-MS），快原子轰击质谱（FAB-MS），红外（IR），紫外（UV）分光光度法进行了鉴定。讨论了麦内酯A和B的H-1 NMR全归属和麦黄酮的C-13 NMR全归属。据我们所知，hentriacomane，1-tetratriacontanol，和β-谷甾醇-3-O-β-D-葡萄糖苷首次在稻壳中鉴定。在使用这些鉴定的化合物的生物活性测试中，豆内酯A和B显示出对浮萍（浮萍）的有效抑制活性。1-三十烷醇和β-谷甾醇-β-O-β-D-葡萄糖苷也显示出基于叶绿素减少的约13-20%的抑制活性。三十一烷和β-谷甾醇未显示出任何除草活性。在三种杂草物种（中国纤蕨（Leptotera chinenesis L.），反枝苋，和Cyperus difformis L.）在培养管中，毛霉内酯A和B均具有较高的抑制作用。在20 ppm时，豆蔻内酯B完全抑制所有三种杂草的萌发。L. Chinensis L.被4 ppm的momilactone B溶液完全抑制。
An ethyl acetate extract of Oryza sativa (rice) hulls yielded seven compounds: hentriacomane, 1-tetratriacontanol, beta-sitosterol, momilactone A, momilactone B, tricin (a flavonoid), and beta-sitosterol-3-O-beta-D-glucoside. The structures of these compounds were elucidated with 500 MHz nuclear magnetic resonance (NMR), using 1D and 2D spectral methods, aided by electron ionization mass spectrometry (EI-MS), fast atom bombardment mass spectrometry (FAB-MS), infrared (IR), and ultraviolet (UV) spectrophotometry. The complete H-1 NMR assignments for momilactone A and B and C-13 NMR assignments for tricin are discussed. To the best of our knowledge, hentriacomane, 1-tetratriacontanol, and beta-sitosterol-3-O-beta-D-glucoside were identified for the first time in rice hulls. In biological activity tests using these identified compounds, momilactone A and B showed potent inhibitory activity against duckweed (Lemna paucicostata). 1-Tetratriacontanol and beta-sitosterol-beta-O-beta-D-glucoside also showed about 13-20% inhibitory activity based on chlorophyll reduction. Hentriacontane and beta-sitosterol did not show any herbicidal activity. In a germination assay of three weed species (Leptochloa chinenesis L., Amaranthus retroflexus L., and Cyperus difformis L.) in culture tubes both momilactones A and B had high inhibitory effects. Momilactone B completely inhibited germination of all three weed species at 20 ppm. Germination of L. chinensis L. was completely inhibited by a 4 ppm solution of momilactone B.