A new visualization approach for identifying mutations that affect differentiation and organization of the Drosophila ommatidia.
A new visualization approach for identifying mutations that affect differentiation and organization of the Drosophila ommatidia.
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发表时间：
2001-03
期刊：
影响因子：
4.6
通讯作者：
Franck Pichaud;Claude Desplan
中科院分区：
文献类型：
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作者：
Franck Pichaud;Claude Desplan
果蝇眼被广泛用作研究神经元分化、存活和轴突投射的模型系统。光感受器的分化始于方正细胞R8的指定，它顺序地将其他光感受器神经元招募到小眼。组成每个小眼的八个光感受器以两种手性形式存在，沿着两个对称轴排列，这种模式代表了一种研究组织极性的范例。我们已经开发了一种荧光透视方法来可视化活动物中不同类型的光感受器和小眼的组织。这使得我们能够进行F(1)遗传筛选，以分离影响光感受器分化、存活或平面极性的突变体。我们使用已知的遗传背景和影响小眼分化、形态或手性的新突变来说明这种检测系统的力量。
The Drosophila eye is widely used as a model system to study neuronal differentiation, survival and axon projection. Photoreceptor differentiation starts with the specification of a founder cell R8, which sequentially recruits other photoreceptor neurons to the ommatidium. The eight photoreceptors that compose each ommatidium exist in two chiral forms organized along two axes of symmetry and this pattern represents a paradigm to study tissue polarity. We have developed a method of fluoroscopy to visualize the different types of photoreceptors and the organization of the ommatidia in living animals. This allowed us to perform an F(1) genetic screen to isolate mutants affecting photoreceptor differentiation, survival or planar polarity. We illustrate the power of this detection system using known genetic backgrounds and new mutations that affect ommatidial differentiation, morphology or chirality.