Stromagenesis during tumorigenesis: characterization of tumor-associated fibroblasts and stroma-derived 3D matrices.
Stromagenesis during tumorigenesis: characterization of tumor-associated fibroblasts and stroma-derived 3D matrices.
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DOI：
10.1007/978-1-59745-413-1_19
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Cukierman, Edna
中科院分区：
文献类型：
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作者：
Castello-Cros, Remedios;Cukierman, Edna
越来越多的人认识到，癌细胞及其周围基质之间的相互作用对于促进肿瘤的生长和侵袭性至关重要。例如，癌细胞通过在早期肿瘤发展期间增加成纤维基质细胞的旁分泌调节来改变基质细胞外基质的形貌和分子组成。反过来，基质的这些物理和生物化学改变深刻地影响癌细胞的性质。然而，对基质和癌细胞之间的串扰知之甚少，这主要是由于缺乏合适的体外系统来模拟体内基质。我们提出了一个体内样的3-D基质系统，来自成纤维细胞收获的组织样本代表不同阶段的基质进展过程中肿瘤发生。本章介绍了如何从大量的组织样本中分离和鉴定成纤维细胞。它描述了如何产生和表征成纤维细胞衍生的3-D矩阵。最后，它描述了如何通过分析在各种3-D矩阵内培养的癌细胞的形态来测试矩阵容许性。
It is increasingly recognized that interactions between cancer cells and their surrounding stroma are critical for promoting the growth and invasiveness of tumors. For example, cancer cells alter the topography and molecular composition of stromal extracellular matrix by increasing paracrine regulation of fibroblastic stromal cells during early tumor development. In turn, these physical and biochemical alterations of the stroma, profoundly affect the properties of the cancer cells. However, little is known about the cross-talk between stroma and cancer cells and it is mainly due to the lack of a suitable in vitro system to mimic the stroma in vivo. We present an in vivo-like 3-D stromal system derived from fibroblasts harvested from tissue samples representing various stages of stroma progression during tumorigenesis. The chapter describes how to isolate and characterize fibroblasts from a plethora of tissue samples. It describes how to produce and characterize fibroblast-derived 3-D matrices. Finally, it describes how to test matrix permissiveness by analyzing the morphology of cancer cells cultured within various 3-D matrices.